FNDC4 Protein (AA 41-163, AA 45-167) (Fc Tag)

Catalog No. ABIN7566145

Product Details

Target: FNDC4 (Fibronectin Type III Domain Containing 4 (FNDC4))

Protein Type: Recombinant

Protein Characteristics: AA 41-163, AA 45-167

Origin: Human, Dog, Monkey, Mouse, Rat

Source: HEK-293 Cells

Purification tag / Conjugate: This FNDC4 protein is labelled with Fc Tag.

Purpose: Fc (human):FNDC4 (rec.)

Cross-Reactivity: Dog, Human, Monkey, Mouse, Rat

Characteristics: FNDC4 (extracellular domain, human (aa 45-167) / mouse (aa 41-163)) is fused at the N-terminus to the Fc portion of human IgG1. FNDC4 extracellular domain has 100 % identity between human, mouse, rat, dog and monkey.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 1 mg/mL after reconstitution

Concentration: 1 mg/mL

Buffer: Contains PBS.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: FNDC4 (Fibronectin Type III Domain Containing 4 (FNDC4))

Alternative Name: FNDC4 (FNDC4 Products)

Background:

Fibronectin Type III Domain-containing Protein 4, Fibronectin Type III Repeat-containing Protein 1, Fc Soluble FNDC4, FcsFNDC4

Irisin is a recently described exercise-induced hormone secreted by skeletal muscle in mice and humans. Irisin activates beige fat cells (beige cells have a gene expression pattern distinct from either white or brown fat and are preferentially sensitive to the polypeptide hormone irisin). Irisin is cleaved from the type I membrane protein FNDC5 and improves systemic metabolism by increasing energy expenditure. FNDC4 is an ortholog of FNDC5 with 50 % identity and 86 % similarity compared to Irisin. FNDC4 as well as FNDC5 are extremely well conserved between species. The human FNDC4 gene is highly enriched in liver, brain tissue and adipocytes. FNDC4 is a factor with direct therapeutic potential in inflammatory bowel disease and possibly other inflammatory diseases. Recently, a new role of FNDC4 as a hepatokine has been published. Liver primarily controls the circulating levels of FNDC4 showing tight correlation with insulin sensitivity. In addition, a new orphan adhesion G protein-coupled receptor 116 (GPR116) has been identified as a receptor of FNDC4 in white adipose tissue (WAT), thereby establishing an endocrine FNDC4-GPR116 axis in the control of systemic glucose homeostasis. Moreover, the FNDC4-GPR116 axis is impaired in diabetic patients and therapeutic injections of recombinant Fc-FNDC4 into pre-diabetic mice corrected pre-diabetic hyperglycemia.

Molecular Weight: ~55-60kDa

UniProt: Q9H6D8