IL1RN Protein (AA 27-178, Monomer) (Fc Tag)

Catalog No. ABIN7566395

Product Details

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Protein Type: Recombinant

Protein Characteristics: AA 27-178, Monomer

Origin: Human, Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This IL1RN protein is labelled with Fc Tag.

Purpose: Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.)

Cross-Reactivity: Mouse

Characteristics: The extracellular domain of mouse IL-1Ra (aa 27-178) is fused to the C-terminus of the Fc (LALA-PG) Knob region of human IgG1. Fc (LALA-PG) Knobs:IL-1Ra (mouse) and Fc (LALA-PG) Holes form the Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (rec.) using the Knobs-into-Holes technology (see reference: J.B. Ridgway, et al., Protein Eng. 9, 617 (1996)).

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.005 EU/μg purified protein (LAL test).

Grade: Animal-Free

Biological Activity Comment: It inhibits the binding of IL-1alpha and IL-1beta to the receptor IL-1R. The Fc contains the mutations LALA-PG that abolish the interaction between the Fc and FcgammaRs and therefore Fc undesirable effects.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 1 mg/mL after reconstitution.

Concentration: 1 mg/mL

Buffer: Contains PBS

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0. 1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Alternative Name: IL-1Ra (IL1RN Products)

Background:

Fc (LALA-PG)-KIH:Interleukin-1 Receptor Antagonist (mouse), IL-1Ra, Interleukin-1R Antagonist

IL-1 is a group of inflammatory cytokines, represented by interleukin-1alpha and interleukin-1beta. IL-1Ra is a cytokine initially isolated from macrophages. It shares 19 % of homologous amino acids with IL1A and 30 % of homologous amino acids with IL1B. IL-1Ra is abundant in the proximal digestive tract reproductive system, skin, bone marrow, and liver. IL-1Ra is the principal endogenous IL-1 antagonist. IL-1Ra is upregulated during host acute or chronic inflammatory responses. IL-1Ra plays diverse roles in anti-inflammatory and anti-tumor processes, either inhibiting tumor angiogenesis and proliferation or suppressing tumor migration and metastasis. The protein Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.) is produced by using two different vectors, one encoding for the Fc Knobs (LALA-PG) (human):IL-1Ra (mouse) sequence (synthesizing a protein of 50 kDa) and one encoding for the Fc Holes (LALA-PG) sequence (synthesizing a protein of 30 kDa). Both vectors transfected into HEK293 cells produce both Fc molecules (Knobs-into-Holes technology, J.B. Ridgway, et al., Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein Fc (LALA-PG)-KIH (human):IL-1Ra (mouse) (monomeric) (rec.). This Fc-KIH format allows our mouse IL-1Ra protein to form a monomer that is the most active structure to bind and inhibit the IL-1 receptor. The LALA-PG mutations inhibit binding to FcgammaRs and C1q while FcRn binding and Fc stability remain unaffected. InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research

Molecular Weight: ~50kDa and 30 kDa (SDS-PAGE)

Pathways: NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints