KRT8 Protein
Quick Overview for KRT8 Protein (ABIN935280)
Target
See all KRT8 ProteinsProtein Type
Origin
Source
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Characteristics
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Purified native Bovine Cytokeratin 8 protein
Protein Source: Bovine liver
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Keratin 8 (KRT8) (AA 1-483) protein (His tag)
custom-made KRT8 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Keratin 8 (KRT8) (AA 1-490) protein (His tag)custom-made KRT8 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Keratin 8 (KRT8) (AA 1-483) protein (GST tag)KRT8 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Keratin 8 (KRT8) (AA 1-483) protein (His-IF2DI Tag)KRT8 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute with 250 µL of distilled water.
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Buffer
- Final solution contains 10 mM Tris-HCL buffer pH 8.0, 9 M urea, 9 M EDTA, 9 M DTT, and 10 mM methylammonium chloride.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- KRT8 (Keratin 8 (KRT8))
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Alternative Name
- Cytokeratin 8
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Background
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Keratin, type I cytoskeletal 8 also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that in human is encoded by the KRT8 gene. It is often paired with keratin 18. Antibodies to CK8 (e.g. CAM 5.2) can be used to differentiate lobular carcinoma of the breast from ductal carcinoma of the breast. Keratin 8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.
Description: Bovine liver.
Alternative Names: Cytokeratin 8, Cytokeratin-8 protein, Cytokeratin 8, Cytokeratin-8, Cytokeratin 8 protein, Keratin K8 protein
Target
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