anti-Human Nucleolin antibody for Cytometry by Time of Flight

Recommended Nucleolin Antibody (supplied by: Log in to see )

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Reactivity
Human
233
56
38
12
11
11
10
10
7
7
6
5
3
1
1
1
1
1
1
1
Host
Mouse
125
116
1
Clonality
Monoclonal
Conjugate
This Nucleolin antibody is un-conjugated
16
11
10
9
9
9
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
218
135
125
91
63
58
41
24
21
17
13
9
2
1
1
Options
Supplier
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Supplier Product No.
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Catalog No. ABIN4341002
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
-11.159155 ABIN4341006 CyTOF FACS IF IHC (p) WB Mouse IgG Log in to see 364-5-NCL-902 0
-11.159155 ABIN4341004 CyTOF FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see 364-5 0

General

Antigen Nucleolin (NCL) Antibodies
Reactivity Human
(233), (56), (38), (12), (11), (11), (10), (10), (7), (7), (6), (5), (3), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(125), (116), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Nucleolin antibody is un-conjugated
(16), (11), (10), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(218), (135), (125), (91), (63), (58), (41), (24), (21), (17), (13), (9), (2), (1), (1)
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Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling Images
Specificity Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
No Cross-Reactivity Mouse (Murine), Cow (Bovine), Rat (Rattus)
Purification Protein A or G purified
Immunogen Recombinant human NCL protein
Clone NCL-902
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background Gene Symbol: NCL
Molecular Weight Theoretical MW: 76 kDa
Gene ID 4691
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling Images back to top
Application Notes Western Blot : 0.5 - 1.0 μg/mL, Flow Cytometry : 0.5 - 1 μg/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 μg/mL, Immunofluorescence : 0.5 - 1.0 μg/mL, CyTOF-readyHu-chromosome location: 2q37.1 Immunohistochemistry-Paraffin 0.5 - 1.0 μg/mL for 30 minutes at RT, Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: No Preservative
Preservative Without preservative
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.