anti-Plant Nucleolin antibody for Simple Western

Recommended Nucleolin Antibody

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Reactivity
Human, Mouse (Murine), Plant, Rat (Rattus)
231
62
38
12
11
11
10
10
7
7
6
5
3
1
1
1
1
1
1
1
Host
Rabbit
122
116
1
Clonality
Polyclonal
Conjugate
This Nucleolin antibody is un-conjugated
15
10
9
9
9
8
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunomicroscopy (IM), Simple Western (SimWes), Western Blotting (WB)
210
132
125
89
63
58
39
24
21
17
13
9
2
1
1
Options

Available images

Catalog No. ABIN153181
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General

Antigen Nucleolin (NCL) Antibodies
Reactivity Human, Mouse (Murine), Plant, Rat (Rattus)
(231), (62), (38), (12), (11), (11), (10), (10), (7), (7), (6), (5), (3), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(122), (116), (1)
Clonality
Polyclonal
Conjugate This Nucleolin antibody is un-conjugated
(15), (10), (9), (9), (9), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunomicroscopy (IM), Simple Western (SimWes), Western Blotting (WB)
(210), (132), (125), (89), (63), (58), (39), (24), (21), (17), (13), (9), (2), (1), (1)
Pubmed 19 references available
Supplier

Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling References for anti-Nucleolin antibody (ABIN153181) Images
Purification Unpurified
Immunogen A fusion protein containing amino acids 284-709 of human Nucleolin. [UniProt# P19338]
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling References for anti-Nucleolin antibody (ABIN153181) Images back to top
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background Gene Symbol: NCL
Molecular Weight Theoretical MW: 106 kDa
Gene ID 4691
UniProt P19338
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling References for anti-Nucleolin antibody (ABIN153181) Images back to top
Application Notes Western Blot 1:4000, Simple Western 1:400, Chromatin Immunoprecipitation, Immunohistochemistry 1:50-1:100, Immunocytochemistry/Immunofluorescence 1:50-1:100, Immunohistochemistry-Paraffin 1:50-1:100, Immunomicroscopy, Electron Microscopy 1:10-1:500This Nucleolin antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Electron Microscopy. Chromatin Immunoprecipitation was reported in scientific literature. By Western Blot, a band at approx. 106 kDa represents human Nucleolin. Heat mediated antigen retrieval is recommended for IHC-P. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Nucleolin Antibody IHC-FFPE sections I. Deparaffinization: A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. II. Quench Endogenous Peroxidase: A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details References for anti-Nucleolin antibody (ABIN153181) Images back to top
Format Liquid
Buffer Whole antisera
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-Nucleolin antibody (ABIN153181)

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling Images back to top
Product cited in:

Hoffmann, Umbreit, Krüger, Pelzel, Ernst, Kniemeyer, Guntinas-Lichius, Berndt, von Eggeling: "Identification of Proteomic Markers in Head and Neck Cancer Using MALDI-MS Imaging, LC-MS/MS, and Immunohistochemistry." in: Proteomics. Clinical applications, Vol. 13, Issue 1, pp. e1700173, 2019 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Human).

Banerjee, Aponte-Diaz, Yeager, Sharma, Ning, Oh, Han, Umeda, Hara, Wang, Cameron: "Hijacking of multiple phospholipid biosynthetic pathways and induction of membrane biogenesis by a picornaviral 3CD protein." in: PLoS pathogens, Vol. 14, Issue 5, pp. e1007086, 2018 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Robijns, Molenberghs, Sieprath, Corne, Verschuuren, De Vos: "In silico synchronization reveals regulators of nuclear ruptures in lamin A/C deficient model cells." in: Scientific reports, Vol. 6, pp. 30325, 2016 Further details: Western Blotting

Corne, Sieprath, Vandenbussche, Mohammed, Te Lindert, Gevaert, Gabriele, Wolf, De Vos: "Deregulation of focal adhesion formation and cytoskeletal tension due to loss of A-type lamins." in: Cell adhesion & migration, pp. 1-17, 2016 Method employed by authors: Western Blotting (WB)

Park, Park, Yu, An, Ko, Kim: "Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay." in: Genes & development, Vol. 30, Issue 18, pp. 2093-2105, 2016 (Sample species: Human).

Leaderer, Cashman, Kumar-Singh: "Topical application of a G-Quartet aptamer targeting nucleolin attenuates choroidal neovascularization in a model of age-related macular degeneration." in: Experimental eye research, Vol. 140, pp. 171-8, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Vermezovic, Adamowicz, Santarpia, Rustighi, Forcato, Lucano, Massimiliano, Costanzo, Bicciato, Del Sal, dAdda di Fagagna: "Notch is a direct negative regulator of the DNA-damage response." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 417-24, 2015 (Sample species: Human). Further details: Western Blotting

Sieprath, Corne, Nooteboom, Grootaert, Rajkovic, Buysschaert, Robijns, Broers, Ramaekers, Koopman, Willems, De Vos: "Sustained accumulation of prelamin A and depletion of lamin A/C both cause oxidative stress and mitochondrial dysfunction but induce different cell fates." in: Nucleus (Austin, Tex.), Vol. 6, Issue 3, pp. 236-46, 2015 Method employed by authors: Immunohistochemistry (IHC)

Stępiński: "Nucleolin level in plant root meristematic cells under chilling stress and recovery." in: Micron (Oxford, England : 1993), Vol. 43, Issue 8, pp. 870-5, 2012 (Sample species: Plant). Further details: Immunomicroscopy

Litchfield, Riggs, Hockenberry, Oliver, Barnhart, Cai, Pierce, Ivanova, Bates, Appana, Datta, Kulesza, McBryan, Young, Klinge: "Identification and characterization of nucleolin as a COUP-TFII coactivator of retinoic acid receptor β transcription in breast cancer cells." in: PLoS ONE, Vol. 7, Issue 5, pp. e38278, 2012 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Kume, Iizumi, Shimada, Ito, Kishi, Yamaguchi, Handa: "Role of N-end rule ubiquitin ligases UBR1 and UBR2 in regulating the leucine-mTOR signaling pathway." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 15, Issue 4, pp. 339-49, 2010 (Sample species: Rat (Rattus)). Further details: Western Blotting

Levitt, Clark, Jenkins, Martens, Traynor: "Differential effect of membrane cholesterol removal on mu- and delta-opioid receptors: a parallel comparison of acute and chronic signaling to adenylyl cyclase." in: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22108-22, 2009

González, Guo, Hurley, Sun: "Identification and characterization of nucleolin as a c-myc G-quadruplex-binding protein." in: The Journal of biological chemistry, Vol. 284, Issue 35, pp. 23622-35, 2009 (Sample species: Human). Further details: Chromatin Immunoprecipitation

Drygin, Siddiqui-Jain, OBrien, Schwaebe, Lin, Bliesath, Ho, Proffitt, Trent, Whitten, Lim, Von Hoff, Anderes, Rice: "Anticancer activity of CX-3543: a direct inhibitor of rRNA biogenesis." in: Cancer research, Vol. 69, Issue 19, pp. 7653-61, 2009

Jin, Li, Davis, Zhu, Wang, Pan, Zhang: "Identification of novel proteins associated with both alpha-synuclein and DJ-1." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 5, pp. 845-59, 2007 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Fatemi, Folsom, Reutiman, Sidwell: "Viral regulation of aquaporin 4, connexin 43, microcephalin and nucleolin." in: Schizophrenia research, Vol. 98, Issue 1-3, pp. 163-77, 2007

Novoselov, Kryukov, Xu, Carlson, Hatfield, Gladyshev: "Selenoprotein H is a nucleolar thioredoxin-like protein with a unique expression pattern." in: The Journal of biological chemistry, Vol. 282, Issue 16, pp. 11960-8, 2007 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Huddleson, Ahmad, Lingrel: "Up-regulation of the KLF2 transcription factor by fluid shear stress requires nucleolin." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15121-8, 2006 Further details: Chromatin Immunoprecipitation

Hanakahi, Dempsey, Li, Maizels: "Nucleolin is one component of the B cell-specific transcription factor and switch region binding protein, LR1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 8, pp. 3605-10, 1997 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Images

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling References for anti-Nucleolin antibody (ABIN153181) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Nucleolin (NCL) antibody (ABIN153181) In-situ immunofluorescent staining of PD31 murine pre-B cells using ABIN153181.
Western Blotting (WB) image for anti-Nucleolin (NCL) antibody (ABIN153181) anti-Nucleolin (NCL) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) antibody (ABIN153181) Detection of Nucleolin in human tonsil germinal centers using ABIN153181.
Simple Western (SimWes) image for anti-Nucleolin (NCL) antibody (ABIN153181) Simple Western: Nucleolin Antibody [ABIN153181] - Simple Western lane view shows a sp...
Immunofluorescence (IF) image for anti-Nucleolin (NCL) antibody (ABIN153181) Immunocytochemistry/Immunofluorescence: Nucleolin Antibody [ABIN153181] - IF analysis...
Immunocytochemistry (ICC) image for anti-Nucleolin (NCL) antibody (ABIN153181) Immunocytochemistry: Nucleolin Antibody - Human prostate epithelial cells stained wi...