anti-Rat (Rattus) Nucleolin antibody for Western Blotting

Recommended Nucleolin Antibody

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Epitope
C-Term
14
14
11
10
9
8
7
7
7
7
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus), Dog (Canine)
230
62
37
12
11
10
10
10
7
7
6
5
3
1
1
1
1
1
1
1
Host
Rabbit
122
115
1
Clonality
Polyclonal
Conjugate
This Nucleolin antibody is un-conjugated
15
10
9
9
9
8
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
209
132
124
89
63
58
39
24
21
17
13
9
2
1
1
Options

Available images

Catalog No. ABIN629901
$ 369.29
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
19.89044 ABIN3025707 FACS IF IHC (p) WB Mouse IgG1 NCL-902 0
19.89044 ABIN3025709 FACS IF IHC (p) WB Mouse IgG1 NCL-902 0
19.33695 ABIN153181 ChIP EM ICC IF IHC IHC (p) IM SimWes WB Rabbit Polyclonal 19
16.89044 ABIN2778936 IHC WB Rabbit C-Term Polyclonal 1
13.89044 ABIN2778935 IHC WB Rabbit N-Term Polyclonal 1
13.89044 ABIN2737225 IHC WB Rabbit IgG Polyclonal 0
13.89044 ABIN3048437 IHC WB Rabbit IgG Polyclonal 0
10.89044 ABIN6570190 IHC WB Rabbit IgG Polyclonal 0
7.8904395 ABIN3197743 IHC WB Rabbit Polyclonal 0
7.8904395 ABIN6043718 IHC WB Rabbit IgG full length Polyclonal 0
7 ABIN202879 IHC IHC (p) WB Rabbit IgG C-Term Polyclonal 0
4.8904395 ABIN2462187 IHC ELISA WB Rabbit Polyclonal 0
4.8904395 ABIN6718112 IHC WB Rabbit Polyclonal 0
4 ABIN1386104 IF (p) IHC (p) WB Rabbit IgG Polyclonal 0
1.3369498 ABIN151419 ICC IF IHC (p) IP IHC WB Rabbit C-Term Polyclonal 2
1 ABIN6290705 WB Goat IgG C-Term Polyclonal 0
1 ABIN1417462 IHC (p) WB HRP Rabbit IgG Polyclonal 0
1 ABIN1399591 IHC (p) WB Biotin Rabbit IgG Polyclonal 0
1 ABIN1916332 IF IHC IHC (p) IP WB Rabbit IgG AA 550-710, C-Term Polyclonal 0
1 ABIN1916330 IF IHC IHC (p) IP WB Rabbit IgG AA 250-300 Polyclonal 0

General

Antigen Nucleolin (NCL) Antibodies
Epitope C-Term
(14), (14), (11), (10), (9), (8), (7), (7), (7), (7), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus), Dog (Canine)
(230), (62), (37), (12), (11), (10), (10), (10), (7), (7), (6), (5), (3), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(122), (115), (1)
Clonality
Polyclonal
Conjugate This Nucleolin antibody is un-conjugated
(15), (10), (9), (9), (9), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(209), (132), (124), (89), (63), (58), (39), (24), (21), (17), (13), (9), (2), (1), (1)
Supplier

Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling Images
Specificity Nucleolin antibody was raised against the C terminal of NCL
Purification Purified
Immunogen Nucleolin antibody was raised using the C terminal of NCL corresponding to a region with amino acids GGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Molecular Weight 78 kDa (MW of target protein)
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling Images back to top
Application Notes WB: 1.25 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator.
Comment

Nucleolin Blocking Peptide, catalog no. 33R-3278, is also available for use as a blocking control in assays to test for specificity of this Nucleolin antibody

Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Images back to top
Format Lyophilized
Reconstitution Lyophilized powder. Add distilled water for a 1 mg/mL concentration of NCL antibody in PBS
Concentration Lot specific
Buffer PBS
Handling Advice Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
Storage 4 °C
Storage Comment Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Images

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) (C-Term) antibody (ABIN629901) Nucleolin antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml ...
Western Blotting (WB) image for anti-Nucleolin (NCL) (C-Term) antibody (ABIN629901) Nucleolin antibody used at 1.25 ug/ml to detect target protein.