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|Antigen||Nucleolin (NCL) Antibodies|
|Reactivity||Human, Rat (Rattus), Dog (Canine) Alternatives|
|Conjugate||This Nucleolin antibody is un-conjugated Alternatives|
Immunohistochemistry (IHC), Western Blotting (WB)
Product Details anti-Nucleolin AntibodyTarget Details Nucleolin Application Details Handling Images
|Specificity||Nucleolin antibody was raised against the C terminal of NCL|
|Immunogen||Nucleolin antibody was raised using the C terminal of NCL corresponding to a region with amino acids GGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE|
|Plasmids, Primers & others|
Target Details NucleolinProduct Details anti-Nucleolin Antibody Application Details Handling Images back to top
|Alternative Name||Nucleolin (NCL Antibody Abstract)|
|Background||Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.|
|Molecular Weight||78 kDa (MW of target protein)|
|Pathways||Ribonucleoprotein Complex Subunit Organization|
Application DetailsProduct Details anti-Nucleolin Antibody Target Details Nucleolin Handling Images back to top
WB: 1.25 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator.
Nucleolin Blocking Peptide, catalog no. 33R-3278, is also available for use as a blocking control in assays to test for specificity of this Nucleolin antibody
|Restrictions||For Research Use only|
HandlingProduct Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Images back to top
|Reconstitution||Lyophilized powder. Add distilled water for a 1 mg/mL concentration of NCL antibody in PBS|
Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
|Storage Comment||Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.|
ImagesProduct Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling back to top