Donkey anti-Mouse IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN101700

Supplier Product No.: 610-701-124

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Donkey

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 610-701-124

Supplier: Rockland

Purpose: Mouse IgG (H&L) Antibody Pre-adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.  Specificity was confirmed using ELISA at less than 1.0 % cross reactivity against IgG proteins purified from the species listed above.

Characteristics: Anti-Mouse IgG Antibody generated in donkey detects reactivity to Mouse IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Application Note: Suitable for immunoblotting (western or dot blot), ELISA, electron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Anti-Mouse IgG whole molecule antibody generated in donkey detects specifically Mouse IgG whole molecule . This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.