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Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, IHC, WB, CUT&Tag, CUT&RUN Polyclonal IgG unconjugated
Catalog No. ABIN101785
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2332
    • 499
    • 411
    • 188
    • 129
    • 122
    • 92
    • 29
    • 28
    • 28
    • 15
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 691
    • 672
    • 559
    • 452
    • 366
    • 239
    • 198
    • 147
    • 137
    • 131
    • 122
    • 98
    • 95
    • 95
    • 92
    • 62
    • 46
    • 33
    • 27
    • 17
    • 12
    • 10
    • 8
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 1718
    • 1473
    • 537
    • 203
    • 118
    • 103
    • 33
    • 31
    • 21
    • 17
    • 15
    • 11
    • 11
    • 1
    Rabbit
    Clonality
    • 3350
    • 164
    • 1
    Polyclonal
    Application
    • 1836
    • 1788
    • 1787
    • 1498
    • 962
    • 934
    • 913
    • 515
    • 234
    • 115
    • 115
    • 111
    • 100
    • 77
    • 59
    • 50
    • 50
    • 49
    • 32
    • 27
    • 25
    • 21
    • 17
    • 16
    • 11
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Cleavage Under Targets and Tagmentation (CUT&Tag), Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
    Specificity
    IgG (H&L)
    Cross-Reactivity
    Mouse (Murine)
    Characteristics
    Anti-Mouse antibody generated in rabbit detects specifically mouse IgG (H&L). This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Anti-Mouse IgG was produced by repeated immunization with mouse IgG whole molecule in rabbit

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes
    The rabbit anti-mouse IgG antibody ABIN101785 is suitable for use in ELISA, immunohistochemistry, and Western Blot, CUT&RUN and CUT&Tag. Specific conditions for each assay should be optimized by the end user. General ABIN101785 dilution recommendations for different applications are as follows:
    • ELISA: 1:20,000 - 1:40,000
    • WB: 1:2,000 - 1:10,000
    • IHC: 1:1,000 - 1:5,000
    • CUT&RUN: 1:100
    • CUT&Tag: 1:100
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Expiry Date
    12 months
  • Fujiwara, Tanno, Sugishita, Kishi, Makino, Okada: "Preparation of optimized concanavalin A-conjugated Dynabeads® magnetic beads for CUT&Tag." in: PLoS ONE, Vol. 16, Issue 11, pp. e0259846, (2021) (PubMed).

    Willcockson, Healton, Weiss, Bartholdy, Botbol, Mishra, Sidhwani, Wilson, Pinto, Maron, Skalina, Toro, Zhao, Lee, Hou, Yusufova, Meydan, Osunsade, David, Cesarman, Melnick, Sidoli, Garcia, Edelmann et al.: "H1 histones control the epigenetic landscape by local chromatin compaction. ..." in: Nature, (2020) (PubMed).

    Vonk, van Dooremalen, Liv, Klumperman, Coffer, Saris, Lorenowicz: "Mesenchymal Stromal/stem Cell-derived Extracellular Vesicles Promote Human Cartilage Regeneration In Vitro." in: Theranostics, Vol. 8, Issue 4, pp. 906-920, (2019) (PubMed).

    Kooijmans, Gitz-Francois, Schiffelers, Vader: "Recombinant phosphatidylserine-binding nanobodies for targeting of extracellular vesicles to tumor cells: a plug-and-play approach." in: Nanoscale, Vol. 10, Issue 5, pp. 2413-2426, (2019) (PubMed).

  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background

    Synonyms: rabbit anti-Mouse IgG Antibody, Rabbit-a-Mouse IgG (H&L), Mouse IgG Antibody in rabbit

    Background: Anti-Mouse IgG antibody generated in rabbit detects specifically mouse IgG (H&L). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This anti-Mouse secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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