Mouse anti-Human IgG (Fc Region) Antibody (HRP)
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- Target See all IgG products
- IgG
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Binding Specificity
- Fc Region
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- HRP
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Application
- ELISA, Western Blotting (WB)
- Purpose
- Anti-Hu IgG (Fc) HRP
- Specificity
- The mouse monoclonal antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
- Cross-Reactivity (Details)
- Human, Other not determined
- Purification
- Purified antibody is conjugated with activated horseradish peroxidase (HRP) of high specific enzyme activity under optimum conditions and unconjugated antibody and free HRP are removed by size-exclusion chromatography.
- Immunogen
- Fusion protein of human IgG Fc fragment.
- Clone
- EM-07
- Isotype
- IgG1
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- Application Notes
- Western blotting and ELISA: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments. The antibody EM-07 does not crossreact with IgM.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01 % thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze.
Avoid prolonged exposure to light. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.,Immunoglobulin G Fc fragment
- Molecular Weight
- 150 kDa
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