Donkey anti-Rabbit IgG (Fc Region) Antibody (Biotin)
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- Target See all IgG products
- IgG
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Binding Specificity
- Fc Region
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Reactivity
- Rabbit
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Host
- Donkey
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 611-706-003
- Supplier
- Rockland
- Purpose
- Rabbit IgG Fc Antibody Biotin Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab).
- Characteristics
- Anti-Rabbit IgG (H&L) Alkaline Phosphatase Antibody generated in donkey detects reactivity to Rabbit IgG.
- Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Immunogen
- Optional[Immunogen]: Rabbit IgG F(c) fragment
- Isotype
- IgG
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Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)High-quality product from Rockland ELISA, IHC, WB, DB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 800)ELISA, WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Rabbit IgG antibody (DyLight 488)WB, IF, FLISA, FM, DB, MA Polyclonal IgG DyLight 488
Goat anti-Rabbit IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
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- Application Notes
- Application Note: Anti-Rabbit IgG F(c) Biotin antibody has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:300,000 Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- Concentration
- 2.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
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