Mouse anti-Human Bence-Jones Protein kappa (Chain kappa), (Free & Bound) Antibody
ELISA, WB
Monoclonal
NI 285 and NI 250
IgG1 kappa
unconjugated
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- Target
- Bence-Jones Protein kappa
- Binding Specificity
- Chain kappa, Free & Bound
- Reactivity
- Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- ELISA, Western Blotting (WB)
- Characteristics
-
The reactivity of the this preparation of two monoclonal antibodies is restricted to polyclonal and monoclonal immunoglobulins of the kappa type, as well as free kappa light chains as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immuno-peroxidase staining. Purified monoclonal mouse antibody to human Ig kappa chain, free and bound
- Purification
- Purified
- Immunogen
- Highly purified Bence Jones kappa proteins isolated from pooled human urine.
- Clone
- NI 285 and NI 250
- Isotype
- IgG1 kappa
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- Application Notes
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To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in indirect techniques as ELISA, indirect immunoperoxidase staining and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
- Concentration
- 0.4 mg/mL
- Buffer
- Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
- Preservative
- Without preservative
- Handling Advice
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- Target
- Bence-Jones Protein kappa
- Alternative Name
- Bence Jones kappa
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