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Goat anti-Human IgG (Fc Region) Antibody

ELISA, WB, IF, DB Polyclonal unconjugated
Catalog No. ABIN458226
  • Target See all IgG products
    IgG
    Binding Specificity
    • 2398
    • 515
    • 417
    • 146
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 1
    • 1
    Fc Region
    Reactivity
    • 687
    • 677
    • 573
    • 433
    • 381
    • 208
    • 195
    • 144
    • 127
    • 126
    • 107
    • 102
    • 96
    • 88
    • 82
    • 58
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 1706
    • 1431
    • 525
    • 205
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Goat
    Clonality
    • 3131
    • 172
    • 16
    • 1
    Polyclonal
    Application
    • 2039
    • 1985
    • 1823
    • 1779
    • 998
    • 920
    • 896
    • 487
    • 424
    • 149
    • 98
    • 64
    • 56
    • 51
    • 45
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 8
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)
    Specificity
    Specificity Reference Reagent. Purified IgG fraction of polyclonal Goat antiSerum to Human IgG, Fc specific.
    Cross-Reactivity (Details)
    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in immunoelectrophoresis a reaction with Rhesus Monkey, Cynomolgus and Baboon has been found.
    Characteristics
    The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule including all subclasses. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal human plasma and serum, a characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG. No reaction is obtained with purified IgA, IgM and IgG/Fab fragments or any other serum protein. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
    Purification
    Purified
    Immunogen
    Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
  • Application Notes
    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
    Buffer
    Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
    Preservative
    Without preservative
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
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