Goat anti-Rat IgA, IgG, IgM Antibody (HRP)
Rockland 612-103-130
ELISA, IHC, WB
Polyclonal
IgG
HRP
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- Target See all IgA, IgG, IgM products
- IgA, IgG, IgM
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Reactivity
- Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- HRP
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 612-103-130
- Supplier
- Rockland
- Purpose
- Rat IgG IgA IgM (H&L) Antibody Peroxidase Conjugated
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. This reagent is suitable for the detection of all Rat immunoglobulin isotypes and chain combinations.
- Characteristics
- Anti-Rat IgG whole molecule antibody generated in goat detects specifically Rat IgG whole molecule. This secondary antibody anti-Rat is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
- Purification
- Anti-Rat IgG IgA IgM (H&L) Antibody, Peroxidase Conjugated, was prepared from polyspecific antiserum by immunoaffinity chromatography using Rat antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Immunogen
- Optional[Immunogen]: Rat IgG, IgA and IgM whole molecules
- Isotype
- IgG
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-
- Application Notes
- Application Note: Anti-Rat IgG IgA IgM Antibody Peroxidase Conjugated has been tested by ELISA and is suitable for immunoassays where reactivity to different classes of rat immunoglobulins is desired. Antibody has been tested by ELISA, western blot, and immunohistochemistry. Optimal concentrations in immunoassays should be determined by the researcher. Immunohistochemistry Dilution: 1:500-1:1,500 Western Blot Dilution: 1:500-1:3,000 ELISA Dilution: 1:100,000 Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
- Concentration
- 2.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Thimerosal - Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- IgA, IgG, IgM
- Alternative Name
- IgG,IgA,IgM (IgA, IgG, IgM Products)
- Target Type
- Antibody
- Background
- Rat IgG IgA IgM (H&L) Antibody detects immunoglobulin G, A, and M from Rat. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain.
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