Goat anti-Human kappa Light Chain Antibody
Rockland 609-1110
WB, ELISA, IHC
Polyclonal
IgG
unconjugated
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- Target See all kappa Light Chain products
- kappa Light Chain
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 609-1110
- Supplier
- Rockland
- Purpose
- Human (kappa chain) Antibody
- Cross-Reactivity (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. Specificity for kappa chain was confirmed by ELISA minimal cross reactivity against other human heavy or lambda light chain isotypes.
- Characteristics
- Anti-Human IgM (mu heavy chain) generated in goat detects specifically Human IgM mu heavy chain.
- Purification
- Human κ (kappa chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Sterility
- Sterile filtered
- Immunogen
- Optional[Immunogen]: Human kappa light chain
- Isotype
- IgG
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- Application Notes
- Application Note: Anti-Human κ antibody has been tested by ELISA and western blot and is suitable for immunohistochemistry and immunoassays where specificity to the immunoglobulin kappa subunit is desired. Optimal concentrations in immunoassays should be determined by the researcher. Immunohistochemistry Dilution: 1:500 - 1:2,000 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:20,000 Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- Expiry Date
- 12 months
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- Target
- kappa Light Chain
- Abstract
- kappa Light Chain Products
- Background
- The anti-Human κ (kappa chain) Antibody detects the kappa chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain.
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