Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN964853

Supplier Product No.: 310-1302

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 310-1302

Supplier: Rockland

Purpose: F(ab')2 Mouse IgG (H&L) Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against anti-Goat IgG F(c) or anti-Pepsin.

Characteristics: F(ab')2 Anti-Mouse IgG (H&L) Fluorescein Antibody generated in goat was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Purification: This product is a F(ab')2 fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.

Immunogen: Optional[Immunogen]: Mouse IgG whole molecule

Isotype: IgG

Application Details

Application Notes: Application Note: Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.