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anti-Eukaryotic Translation Initiation Factor 4E (EIF4E) Antibodies

The protein encoded by EIF4E is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. Additionally we are shipping EIF4E Proteins (18) and EIF4E Kits (2) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
EIF4E 1977 P06730
EIF4E 13684 P63073
EIF4E 117045 P63074
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Top anti-EIF4E Antibodies at antibodies-online.com

Showing 10 out of 237 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated IF (cc), IF (p), IHC (p), WB 100 μL Log in to see 3 to 7 Days
$269.50
Details
Cow Rabbit Un-conjugated IHC, WB WB Suggested Anti-EIF4E Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human brain Sample Type: Lane1 = 10ug HEK293 lysateLane 2 = 50ug HEK293 lysatePrimary Antibody Dilution: Anti-EIF4E 1:1000Submitted By: Dr. Veronique Dorval, Laval University 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated IHC (p), WB Western blot analysis of anti-EIF4E Antibody (N-term) Pab (ABIN388675) in HL60 cell line lysates. EIF4E Antibody (N-term)(arrow) was detected using the purified polyclonal antibody. Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E antibody (N-term)(ABIN388675), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated EIA, IHC (p), WB 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Human Rabbit Un-conjugated EIA 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Human Mouse Un-conjugated FACS, WB EIF4E Antibody  western blot analysis in Jurkat cell line lysates (15ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow). EIF4E Antibody  western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow). 0.1 mL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated DB Dot blot analysis of anti-EIF4E-pS209 Phospho-specific Pab (ABIN389859) on nitrocellulose membrane.  50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/mL. 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated FACS, IHC (p), WB Western blot analysis of EIF4E Antibody (ABIN650675) in HL-60 cell line lysates (35 µg/lane). EIF4E (arrow) was detected using the purified polyclonal antibody. Formalin-fixed and paraffin-embedded human testis tissue reacted with APOL5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Mouse Un-conjugated IF, WB Fluorescent image of Hela cell stained with EIF4E Antibody.Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EIF4E primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). EIF4E immunoreactivity is localized to Cytoplasm significantly. EIF4E Antibody        western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EIF4E       antibody detected the EIF4E       protein (arrow). 400 μL Log in to see 10 to 11 Days
$324.50
Details
Chicken Mouse Un-conjugated BI, WB Western blot analysis of eIF-4E on a A431 lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the eIF-4E antibody. Immunofluorescent staining of U-2 OS (ATCC HTB-96) cells. Cells were seeded in a 96 well imaging plate at ~ 10 000 cells per well. After overnight incubation, cells were stained using the alcohol perm protocol and the anti-eIF-4E antibody. The second step reagent was Alexa Fluor 488 goat anti mouse Ig (Invitrogen). Images were taken on a BD Pathway™ 855 Bioimager using a 20x objective. This antibody also stained A549 (ATCC CCL-185) and HeLa (ATCC CCL-2) cells. The Triton™ X-100 perm protocol is not recommended for use with this antibody. 50 μg Log in to see 5 to 6 Days
$205.42
Details

EIF4E Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-EIF4E Antibodies

  1. Chicken Monoclonal EIF4E Primary Antibody for BI, WB - ABIN967869 : De Benedetti, Rhoads: Overexpression of eukaryotic protein synthesis initiation factor 4E in HeLa cells results in aberrant growth and morphology. in Proceedings of the National Academy of Sciences of the United States of America 1990 (PubMed)
    Show all 5 references for ABIN967869

  2. Human Polyclonal EIF4E Primary Antibody for DB - ABIN389859 : Rychlik, Russ, Rhoads: Phosphorylation site of eukaryotic initiation factor 4E. in The Journal of biological chemistry 1987 (PubMed)
    Show all 4 references for ABIN389859

  3. Human Polyclonal EIF4E Primary Antibody for EIA - ABIN358406 : Whalen, Gingras, Amankwa, Mader, Branton, Aebersold, Sonenberg: Phosphorylation of eIF-4E on serine 209 by protein kinase C is inhibited by the translational repressors, 4E-binding proteins. in The Journal of biological chemistry 1996 (PubMed)
    Show all 4 references for ABIN358406

  4. Human Polyclonal EIF4E Primary Antibody for EIA, IHC (p) - ABIN357297 : Dorfman, Lazaris-Karatzas, Malo, Sonenberg, Gros: Chromosomal assignment of one of the mammalian translation initiation factor eIF-4E genes. in Genomics 1991 (PubMed)
    Show all 4 references for ABIN357297

  5. Human Polyclonal EIF4E Primary Antibody for IHC (p), WB - ABIN388675 : Pelletier, Brook, Housman: Assignment of two of the translation initiation factor-4E (EIF4EL1 and EIF4EL2) genes to human chromosomes 4 and 20. in Genomics 1991 (PubMed)
    Show all 4 references for ABIN388675

  6. Human Monoclonal EIF4E Primary Antibody for FACS, WB - ABIN659031 : Hoeffer, Cowansage, Arnold, Banko, Moerke, Rodriguez, Schmidt, Klosi, Chorev, Lloyd, Pierre, Wagner, LeDoux, Klann: Inhibition of the interactions between eukaryotic initiation factors 4E and 4G impairs long-term associative memory consolidation but not reconsolidation. in Proceedings of the National Academy of Sciences of the United States of America 2011 (PubMed)
    Show all 3 references for ABIN659031

  7. Cow (Bovine) Polyclonal EIF4E Primary Antibody for IHC, WB - ABIN2778784 : Lee, Cho, Kim: Ectopic expression of eIF4E-transporter triggers the movement of eIF4E into P-bodies, inhibiting steady-state translation but not the pioneer round of translation. in Biochemical and biophysical research communications 2008 (PubMed)

  8. Human Polyclonal EIF4E Primary Antibody for FACS, IHC (p) - ABIN650675 : Kim, Wang, Ryu: Incorporation of eukaryotic translation initiation factor eIF4E into viral nucleocapsids via interaction with hepatitis B virus polymerase. in Journal of virology 2009 (PubMed)

  9. Human Monoclonal EIF4E Primary Antibody for IF, WB - ABIN659032 : Hoeffer, Santini, Ma, Arnold, Whelan, Wong, Pierre, Pelletier, Klann: Multiple components of eIF4F are required for protein synthesis-dependent hippocampal long-term potentiation. in Journal of neurophysiology 2013 (PubMed)

More Antibodies against EIF4E Interaction Partners

Fruit Fly (Drosophila melanogaster) Eukaryotic Translation Initiation Factor 4E (EIF4E) interaction partners

  1. Phosphorylation of 4E-BP1 impairs the compet (show EIF4EBP1 Antibodies)ition with eIF4G for eIF4E binding.

  2. Both eIF4E-1 and eIF4E-3 (show EIF4E3 Antibodies) are required in spermatocytes for chromosome condensation and cytokinesis during the meiotic stages.

  3. eIF4E-binding protein (show EIF4EBP1 Antibodies) requires non-canonical 4E-binding motifs and a lateral surface of eIF4E to repress translation.

  4. Protein-protein interactions rather than interactions with the mRNA are essential for the recruitment of eIF4E and for a putative nucleation function.

  5. Eukaryotic initiation factor 4E-3 is essential for meiotic chromosome segregation, cytokinesis and male fertility in Drosophila.

  6. eIF4E regulates the sex-specific expression of the master switch gene Sxl.

  7. data are consistent with the idea that Parkin (show PARK2 Antibodies) and eIF4E act in a common pathway, likely modulating cap-dependent translation initiation events.

  8. results show that LK6 binds to ERK (show MAPK1 Antibodies) and is activated by ERK (show MAPK1 Antibodies) signalling and it is responsible for phosphorylating eIF4E in Drosophila

  9. our results suggest that the level of eIF4E protein is regulated by Diap1 (show DIAPH1 Antibodies), and that IAPs may play a role in cap-dependent translation by regulating the level of eIF4E protein.

  10. These results suggest that the eIF4E-1,2 gene is regulated by Hfp through a mechanism linked to transcription control and 3' splice site selection, which determines the pattern and translation efficiency of eIF4E-1,2 mRNAs.

Xenopus laevis Eukaryotic Translation Initiation Factor 4E (EIF4E) interaction partners

  1. Pumilio 2 (show PUM2 Antibodies) controls translation by competing with eIF4E for 7-methyl guanosine cap recognition.

  2. CDK1 (show CDK1 Antibodies) and calcineurin (show PPP3CA Antibodies) regulate Maskin (show TACC3 Antibodies) association with eIF4E and translational control of cell cycle progression

  3. CPEB, partnered with several highly conserved RNA-binding partners, inhibits protein synthesis in oocytes using a novel pairing of 4E-T (show EIF4ENIF1 Antibodies) and eIF4E1b (show EIF4E1B Antibodies)

Human Eukaryotic Translation Initiation Factor 4E (EIF4E) interaction partners

  1. This study demonstrates that the activation of eIF4E gene is an essential component of the malignant phenotype in ovarian cancer

  2. This review will present eIF4E's structure and functions. It will also expose the use of eIF4E as a therapeutic target in cancer

  3. mechanistic analysis suggested that LMP1 (show PDLIM7 Antibodies) overexpression induced the expression of eIF4E, while eIF4E-shRNA dramatically attenuated the increase in cell proliferation, invasion, migration and the inhibition of apoptosis triggered by LMP-1 (show PDLIM7 Antibodies) upregulation

  4. Our findings identify the eIF4E- beta-catenin (show CTNNB1 Antibodies) axis as a critical regulator of lung cancer cell growth and survival, and suggest that its pharmacological inhibition may be therapeutically useful in lung cancer.

  5. Activated eIF4E, which is phosphorylated by MNK1 (show MKNK1 Antibodies), is a regulator of Sox2 (show SOX2 Antibodies) expression after irradiation of pancreatic tumor cells.

  6. Findings provide new evidence that PRMT5 (show PRMT5 Antibodies) plays an important role in CRC (show CALR Antibodies) pathogenesis through epigenetically regulating arginine methylation of oncogenes such as eIF4E and FGFR3 (show FGFR3 Antibodies).

  7. Data suggest MNK1 (show MKNK1 Antibodies)/MNK2 (show MKNK2 Antibodies) stimulate mRNA translation but only of mRNA containing both 5-prime-terminal cap and hairpin duplex; this stimulation involves up-regulation of phosphorylation/mRNA un-winding activity of eIF4E (via decreased binding to eIF4G (show EIF4G1 Antibodies)).

  8. Molecular structure of EIF4E bound to 4E-BP1 (show EIF4EBP1 Antibodies) provides insight into the consequences of phosphorylation.Phosphorylation of 4E-BP1 (show EIF4EBP1 Antibodies) impairs the competition with eIF4G (show EIF4G1 Antibodies) for eIF4E binding.

  9. Results suggest that the phosphorylated serine 209 residue of eukaryotic translation initiation factor 4E (p-eIF4E) might be a potential target for developing small molecule-based new therapies for leukemia.

  10. Report the presence of a cryptic pocket at the protein-binding interface of eIF4E, which opens transiently during molecular dynamics simulations of the protein in solvent water and is observed to be stable when water is mixed with benzene molecules.

Mouse (Murine) Eukaryotic Translation Initiation Factor 4E (EIF4E) interaction partners

  1. Results of our study suggest that the eIF4E/Fmr1 (show FMR1 Antibodies) double mutant mouse may be a reliable model to study cognitive dysfunction in the context of autism spectrum disorder.

  2. Our findings identify the eIF4E- beta-catenin (show CTNNB1 Antibodies) axis as a critical regulator of lung cancer cell growth and survival, and suggest that its pharmacological inhibition may be therapeutically useful in lung cancer.

  3. Unphosphorylated HSP27 (show HSPB1 Antibodies) associates with eIF4E in osteoblasts and suppresses the translation initiation process.

  4. Rotenone induction of hydrogen peroxide inhibits mTOR (show FRAP1 Antibodies)-mediated S6K1 (show RPS6KB1 Antibodies) and 4E-BP1 (show EIF4EBP1 Antibodies)/eIF4E pathways, resulting in caspase (show CASP3 Antibodies)-dependent and -independent apoptosis in neuronal cells.

  5. Findings indicate eIF4E is maintained at levels in excess (show RCC1 Antibodies) for normal development that are hijacked by cancer cells to drive a translational program supporting tumorigenesis.

  6. a light- and circadian clock-regulated MAPK (show MAPK1 Antibodies)/MNK (show ATP7A Antibodies) pathway led to phosphorylation of the cap-binding protein eIF4E

  7. mice in which eukaryotic translation initiation factor 4E (eIF4E) cannot be phosphorylated are resistant to lung metastases in a mammary tumor model, and cells isolated from these mice exhibit impaired invasion.

  8. Data suggest that MAP kinase (show MAPK1 Antibodies)-interacting kinases (Mnk1 (show MKNK1 Antibodies), Mnk2 (show MKNK2 Antibodies)) regulate cell migration/wound healing, expression of vimentin (show VIM Antibodies), stability of vimentin (show VIM Antibodies) protein, and binding of eIF4E and Cyfip1 (cytoplasmic FMR1 interacting protein 1 (show CYFIP1 Antibodies)).

  9. results show that the activation of p38 (show CRK Antibodies) and Mnk (show ATP7A Antibodies) during MNV1 infection is important for MNV1 replication. Furthermore, phosphorylated eIF4E relocates to the polysomes, and this contributes to changes in

  10. Maintaining eIF4E levels below its proneoplastic threshold is an important anticancer defense in normal cells, with important implications for understanding pregnancy-associated breast cancer.

Pig (Porcine) Eukaryotic Translation Initiation Factor 4E (EIF4E) interaction partners

  1. These data suggest that sapovirus VPg can hijack the cellular translation initiation mechanism by recruiting the eIF4F (show EIF4A2 Antibodies) complex through a direct eIF4E interaction

  2. it is proposed that a balanced regulation of the truncation of the cap-binding complex component eIF4F (show EIF4A2 Antibodies) and degradation of 4E-BP1 (show EIF4EBP1 Antibodies) and/or truncation of 4E-BP2 (show EIF4EBP2 Antibodies) that together ensures correct translational control during the dynamic process of conceptus implantation

  3. Results show that in pigs, the truncated eIF4E is located in the endometrial luminal epithelium during implantation. Neither glandulary tissue nor stroma expressed any truncated eIF4E.

  4. The translation initiation in the endometrium is differently regulated by the two eIF4E forms with regard to different 4E-BP1 (show EIF4EBP1 Antibodies) abundance and phosphorylation as well as different eIF4E/4E-BP1 (show EIF4EBP1 Antibodies) binding dynamic depending on the type of implantation.

  5. Modified translational initiation of eIF4E may particularly regulate protein synthesis during conceptus attachment at the time of implantation in swine.

  6. Translation initiation factor eIF4E is phosphorylated during in vitro maturation of pig oocytes with a maximum in metaphase II stage oocytes.

EIF4E Antigen Profile

Protein Summary

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene.

Gene names and symbols associated with EIF4E

  • Eukaryotic initiation factor 4E (eIF-4E) antibody
  • eucaryotic initiation factor6 (eif6) antibody
  • eukaryotic translation initiation factor 4E (eif4e) antibody
  • eIF-4E protein (eif4e) antibody
  • eukaryotic translation initiation factor 4E (EIF4E) antibody
  • eukaryotic translation initiation factor 4E (Eif4e) antibody
  • eukaryotic translation initiation factor 4ea (eif4ea) antibody
  • translation initiation factor eIF4E (LOC100533326) antibody
  • 2 antibody
  • 0260/09 antibody
  • 0587/11 antibody
  • 0589/11 antibody
  • 0919/12 antibody
  • 1004/13 antibody
  • 7238 antibody
  • AUTS19 antibody
  • cbp antibody
  • CG4035 antibody
  • CT13384 antibody
  • CT39424 antibody
  • CT39426 antibody
  • D-eIF4E antibody
  • deIF-4E antibody
  • deIF4E antibody
  • Dmel\\CG4035 antibody
  • EG668879 antibody
  • EIF-4E antibody
  • eIF-4E1 antibody
  • eIF-4E2 antibody
  • eIF-4EII antibody
  • eif4e antibody
  • eIF 4E antibody
  • eif4e-1 antibody
  • eIF4E-1/2 antibody
  • eIF4E-2 antibody
  • Eif4e-ps antibody
  • EIF4E1 antibody
  • eIF4EI antibody
  • EIF4EL1 antibody
  • EIF4F antibody
  • If4e antibody
  • l(3)07238 antibody
  • l(3)67Af antibody
  • l(3)S025007 antibody
  • l(3)S026009 antibody
  • l(3)S058711 antibody
  • l(3)S091912 antibody
  • zgc:86680 antibody

Protein level used designations for EIF4E

CG4035-PA , CG4035-PB , CG4035-PC , CG4035-PD , CG4035-PE , CG4035-PF , CG4035-PG , CG4035-PH , CG4035-PI , cap binding protein , eIF-4E-PA , eIF-4E-PB , eIF-4E-PC , eIF-4E-PD , eIF-4E-PE , eIF-4E-PF , eIF-4E-PG , eIF-4E-PH , eIF-4E-PI , eukaryotic initiation factor 4E , eukaryotic translation initiation factor 4E , lethal(3)67Af , eucaryotic initiation factor6 , eIF4E , PCO081734 , PCO081734_ov , mRNA cap-binding protein , eukaryotic translation initiation factor small subunit , eIF-4F 25 kDa subunit , eIF-4E , eukaryotic translation initiation factor 4E-like 1 , elongation initiation factor 4E , eukaryotic translation initiation factor 4e 1a , eukaryotic translation initiation factor eIF4E-1

GENE ID SPECIES
45525 Drosophila melanogaster
542608 Zea mays
548663 Xenopus (Silurana) tropicalis
399255 Xenopus laevis
1977 Homo sapiens
13684 Mus musculus
117045 Rattus norvegicus
422703 Gallus gallus
734259 Xenopus laevis
281751 Bos taurus
100009338 Oryctolagus cuniculus
487870 Canis lupus familiaris
100038030 Sus scrofa
79380 Danio rerio
100533326 Aplysia californica
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