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anti-Secreted phosphoprotein 1 (SPP1) Antibodies

The protein encoded by SPP1 is involved in the attachment of osteoclasts to the mineralized bone matrix. Additionally we are shipping Osteopontin Kits (118) and Osteopontin Proteins (58) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
SPP1 6696 P10451
SPP1 20750 P10923
SPP1 25353 P08721
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Top anti-Osteopontin Antibodies at

Showing 10 out of 428 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Dog Rabbit Un-conjugated EIA, IHC (p), WB Figure 1. Immunohistochemistry. Rabbit anti-Osteopontin was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteoponin is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required. 0.2 mL Log in to see 6 to 8 Days
Dog Rabbit Un-conjugated WB WB Suggested Anti-SPP1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: THP-1 cell lysate 100 μL Log in to see 2 to 3 Days
Human Rabbit Un-conjugated EIA, IF, IHC (p), WB Confocal immunofluorescent analysis of OPN-a/b Antibody (N-term)Cat.-No AP52999PU-N with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue reacted with OPN-a/b Antibody (N-term) followed by peroxidase conjugation of the secondary antibody and DAB staining. 0.4 mL Log in to see 6 to 8 Days
Human Rabbit Un-conjugated FACS, IHC (p), WB Western blot analysis of SPP1 Antibody (C-term) (ABIN390469) in NCI-H460 cell line lysates (35 µg/lane). SPP1 (arrow) was detected using the purified polyclonal antibody. Formalin-fixed and paraffin-embedded human lung carcinoma with SPP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 400 μL Log in to see 10 to 11 Days
Human Goat Un-conjugated ELISA, WB ABIN185425 staining (1µg/ml) of MOLT4 cell lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. 100 μg Log in to see 4 to 5 Days
Cow Rabbit Un-conjugated IHC, WB WB Suggested Anti-SPP1 Antibody Titration: 1.25ug/mlELISA Titer: 1:1562500Positive Control: Jurkat cell lysate 100 μL Log in to see 2 to 3 Days
Human Mouse Un-conjugated ICC, IF, IHC, IHC (fro), IHC (p), IP, SimWes, WB 0.1 mL Log in to see 5 to 7 Days
Human Mouse Un-conjugated ICC, IHC, ELISA, WB Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 ng), Blue: Antigen (100 ng) Figure 2: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using SPP1 mouse mAb with DAB staining. 0.1 mg Log in to see 3 to 4 Days
Human Rabbit Un-conjugated EIA, IHC (p), WB 0.1 mL Log in to see 6 to 8 Days
Mouse Rabbit Un-conjugated IF (cc), IF (p), IHC (p), WB Formalin-fixed and paraffin-embedded rat hippocampus labeled with Rabbit Anti-OPN Polyclonal Antibody, Unconjugated (ABIN723800) at 1:300 followed by conjugation to the secondary antibody Formalin-fixed and paraffin embedded rat shin bone labeled with Anti-Osteopontin Polyclonal Antibody, Unconjugated (ABIN723800) at 1:300 followed by conjugation to the secondary antibody and DAB staining 100 μL Log in to see 3 to 7 Days

SPP1 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
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Top referenced anti-Osteopontin Antibodies

  1. Human Monoclonal Osteopontin Primary Antibody for ICC, IF - ABIN259958 : Kon, Yokosaki, Maeda, Segawa, Horikoshi, Tsukagoshi, Rashid, Morimoto, Inobe, Shijubo, Chambers, Uede: Mapping of functional epitopes of osteopontin by monoclonal antibodies raised against defined internal sequences. in Journal of cellular biochemistry 2002 (PubMed)
    Show all 13 references for ABIN259958

  2. Dog (Canine) Polyclonal Osteopontin Primary Antibody for EIA, IHC (p) - ABIN118005 : Yamasaki, Nair, Bhattacharya, Yamamoto, Kurazono, Takeda: Cryptic appearance of a new clone of Vibrio cholerae serogroup 01 biotype El Tor in Calcutta, India. in Microbiology and immunology 1997 (PubMed)
    Show all 12 references for ABIN118005

  3. Human Polyclonal Osteopontin Primary Antibody for EIA, IHC (p) - ABIN191686 : Frosch, Barvencik, Lohmann, Viereck, Siggelkow, Breme, Dresing, Stürmer: Migration, matrix production and lamellar bone formation of human osteoblast-like cells in porous titanium implants. in Cells, tissues, organs 2002 (PubMed)
    Show all 3 references for ABIN191686

  4. Human Polyclonal Osteopontin Primary Antibody for EIA, IF - ABIN953945 : Weber, Lett, Haubein: Osteopontin is a marker for cancer aggressiveness and patient survival. in British journal of cancer 2010 (PubMed)
    Show all 3 references for ABIN953945

  5. Mouse (Murine) Polyclonal Osteopontin Primary Antibody for IF (cc), IF (p) - ABIN723800 : Zhang, Men, Fu, Shan, Ye, Wu, Tao, Liu, Jiang: Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bones. in Cell and tissue research 2012 (PubMed)
    Show all 3 references for ABIN723800

  6. Human Monoclonal Osteopontin Primary Antibody for ICC, IHC - ABIN1098149 : Liersch, Gerss, Schliemann, Bayer, Schwöppe, Biermann, Appelmann, Kessler, Löwenberg, Büchner, Hiddemann, Müller-Tidow, Berdel, Mesters: Osteopontin is a prognostic factor for survival of acute myeloid leukemia patients. in Blood 2012 (PubMed)
    Show all 2 references for ABIN1098149

  7. Human Polyclonal Osteopontin Primary Antibody for ELISA, WB - ABIN185425 : Kolb, Kleeff, Guweidhi, Esposito, Giese, Adwan, Giese, Büchler, Berger, Friess: Osteopontin influences the invasiveness of pancreatic cancer cells and is increased in neoplastic and inflammatory conditions. in Cancer biology & therapy 2005 (PubMed)

  8. Dog (Canine) Polyclonal Osteopontin Primary Antibody for WB - ABIN2774903 : Emani, Zhang, Guo, Guo, Kuo: RNA stability regulates differential expression of the metastasis protein, osteopontin, in hepatocellular cancer. in Surgery 2008 (PubMed)

  9. Human Polyclonal Osteopontin Primary Antibody for FACS, IHC (p) - ABIN390469 : Agnihotri, Crawford, Haro, Matrisian, Havrda, Liaw: Osteopontin, a novel substrate for matrix metalloproteinase-3 (stromelysin-1) and matrix metalloproteinase-7 (matrilysin). in The Journal of biological chemistry 2001 (PubMed)

  10. Cow (Bovine) Polyclonal Osteopontin Primary Antibody for IHC, WB - ABIN2774904 : Iwata, Awaya, Graf, Kahl, Torok-Storb: Human marrow stromal cells activate monocytes to secrete osteopontin, which down-regulates Notch1 gene expression in CD34+ cells. in Blood 2004 (PubMed)

More Antibodies against Osteopontin Interaction Partners

Human Secreted phosphoprotein 1 (SPP1) interaction partners

  1. Data show that increased osteopontin levels are independently associated with type 1 diabetes mellitus (T1DM) pediatric patients.

  2. High circulating levels of SSP1 (show SENP6 Antibodies) is associated with melanoma.

  3. High SPP1 expression is associated with malignant pleural mesothelioma.

  4. Studies indicate it was unable to detect any osteopontin (OPN) protein in the 3 pancreatic ductal adenocarcinoma (PDA) cell lines studied and neither the quantity nor the intensity of OPN staining correlated with any of the pathologic variables available to us in the PDA tissue microarray analysis.

  5. In conclusion, plasma level of OPN may act as diagnostic adjuvant biomarkers for community-acquired pneumonia (CAP) and further play a role in clinical assessment of the severity of CAP, which could potentially guide the development of treatment strategies.

  6. In patients with proliferative plasma cell disorders, a correlation was determined between VEGF (show VEGFA Antibodies) and interstitial OPN with angiogenic parameters in the monoclonal gammopathy of undetermined significance stage of the disease. In advanced stages, there was a negative correlation between OPN and interstitial OPN with parameters of angiogenesis. Overall survival was significantly shorter for patients with negative OPN.

  7. SPP1 expression is upregulated in human masticatory mucosa during wound healing

  8. High OPN expression is associated with gastric cancer.

  9. Treatment with brefelamide induced Smad4 (show SMAD4 Antibodies) expression, and knockdown of Smad4 (show SMAD4 Antibodies) by RNA interference partially diminished the inhibitory effect of brefelamide on OPN. These results indicate that brefelamide inhibited OPNmediated cell invasion through restoration of the OPN repression by TGFbeta (show TGFB1 Antibodies)/Smad (show SMAD1 Antibodies) signaling

  10. OPN can be used as a possible differential diagnostic biomarker to distinguish between malignant serous ovarian carcinoma and borderline ovarian tumours.

Pig (Porcine) Secreted phosphoprotein 1 (SPP1) interaction partners

  1. These data suggested that methylation in the OPN promoter plays a crucial role in the regulation of OPN expression that we found in cloned pigs genome.

  2. The striking breed differences between hyperprolific Large White and Meishanin pigs of secreted phospoprotein 1 expression in endometrium suggest that SPP1 may be associated with placental efficiency.

  3. Microglia incubated in vitro with different concentrations (0.1 fM-1 nM) of recombinant osteopontin showed increased proliferation at 10 fM.

  4. results of this study reveal faster growth rate and differences in pig productivity according to genotypes of the SPP1 gene

  5. Osteopontin could play an important role in the development of neointimal hyperplasia in venous conduits after coronary artery bypass grafting.

  6. A different MSSCP pattern was shown in osteopontin which indicates that mutation is located in promotor region; the A --> G transitions was identified in two positions -617 and -608

  7. The 3' terminal end of the first intron of porcine SPP1 harbors a C/EBPbeta (show CEBPB Antibodies) binding site and this binding site is negatively affected by the mutant G allele.

  8. in pregnant pigs SPP1 is induced by conceptus estrogen in uterine luminal epithelium and is regulated in a manner coincident with placental progesterone production

  9. osteopontin promotes pathologic mineralization via calcium pyrophosphate dihydrate crystal formation in articular cartilage.

  10. Osteopontin is detected in the majority of germ cells and is involved in spermatogenesis in boar testis.

Mouse (Murine) Secreted phosphoprotein 1 (SPP1) interaction partners

  1. OPN induces oxidative stress via the involvement of mitochondria and NOX-4 (show NOX4 Antibodies). It may affect mitochondrial morphology and integrity, at least in part, via the involvement of BIK (show BIK Antibodies).

  2. Endogenous osteopontin plays a critical role in the prevention of phosphate-induced nephrocalcinosis and vascular calcification in response to high phosphate load.

  3. GATA4 (show GATA4 Antibodies) acts as a negative regulator of Bsp (show KLK6 Antibodies) expression in osteoblasts.

  4. Genetic deletion of osteopontin in TRAMP (show DPT Antibodies) mice skews prostate carcinogenesis from adenocarcinoma to aggressive human-like neuroendocrine cancers.

  5. IFN-gamma (show IFNG Antibodies) regulates hypertensive vascular collagen remodeling induced by ANG II (show AGT Antibodies) and inhibits the differentiation of Th17 cells by suppressing OPN expression and regulating secretion of inflammatory cytokines.

  6. results indicate that autocrine OPN plays a crucial role in HPC expansion, migration, and subsequent oncogenic transformation of HPCs, which may provide a new insight into hepatocarcinogenesis

  7. OPN produced by hematogenous macrophages induces astrocyte process extension toward the infarct border zone, which may contribute to repair of the ischemic neurovascular unit following stroke

  8. During the priming phase of liver regeneration, osteopontin enhances the recruitment of macrophages and neutrophils, and triggers hepatocyte proliferation through Kupffer cell-derived IL-6 (show IL6 Antibodies) release and the downstream activation of Stat3 (show STAT3 Antibodies).

  9. OPN deficiency accelerated the spontaneous development of colitis in mice with disrupted gut (show GUSB Antibodies) microbiota and macrophage phagocytic activity.

  10. mechanical stretch (MS) directly increased OPN protein expression and secretion in vascular smooth muscle cells.

Cow (Bovine) Secreted phosphoprotein 1 (SPP1) interaction partners

  1. This implies that a majority of the acidic residues within OPN must be engaged in calcium interaction under physiological conditions.

  2. Genetic variations in the SPP1 promoter affect gene expression and the level of osteopontin secretion into bovine milk.

  3. Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells.

  4. osteopontin (OPN) can serve as a process-directing agent for the intrafibrillar mineralization of collagen.

  5. Results suggest a mechanism of the interaction of UO2(2+) with bone metabolism and a new role for osteopontin (OPN) as a metal transporter.

  6. Upon induction of luteolysis, SPP1 serves as a signaling molecule to recruit or activate immune cells to facilitate luteal regression and tissue degradation.

  7. OPN strongly reduces the amount of biofilm formed in a well-defined laboratory model of acidogenic dental biofilm.

  8. This study demonstrates that adhesion of isolated neonatal rat osteoclasts in vitro was augmented on bovine milk osteopontin (bmOPN) with post-translational modifications (PTMs (show PTMS Antibodies)) compared to human Escherichia-coli-derived recombinant OPN without PTMs (show PTMS Antibodies).

  9. topographical distribution of both the in vivo and in vitro phosphorylation sites of bone sialoprotein (show CRISP1 Antibodies)

  10. the bovine osteopontin gene has two functionally distinct clusters of haplotypes within the QTL region on chromosome 6

Rabbit Secreted phosphoprotein 1 (SPP1) interaction partners

  1. Infection of urinary tract by Escherichia coli caused higher than normal expression of promoter protein osteopontin and mucosal damage at renal tubular cells.

Osteopontin (SPP1) Antigen Profile

Protein Summary

Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000

Gene names and symbols associated with SPP1

  • secreted phosphoprotein 1 (spp1) antibody
  • secreted phosphoprotein 1 (SPP1) antibody
  • secreted phosphoprotein 1 (Spp1) antibody
  • sphingosine-1-phosphate phosphatase 1 (Sgpp1) antibody
  • sphingosine-1-phosphate phosphatase 1 (SGPP1) antibody
  • 2AR antibody
  • AI463453 antibody
  • Apl-1 antibody
  • BNSP antibody
  • Bsp antibody
  • BSPI antibody
  • Eta antibody
  • ETA-1 antibody
  • OP antibody
  • Opn antibody
  • Opnl antibody
  • OSP antibody
  • Ric antibody
  • SPP antibody
  • Spp-1 antibody
  • SPP1 antibody
  • SPPase1 antibody
  • Spph1 antibody
  • zgc:111821 antibody

Protein level used designations for SPP1

spp1 protein , osteopontin , SPP1/CALPHA1 fusion , early T-lymphocyte activation 1 , nephropontin , osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein , secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) , urinary stone protein , uropontin , SPP-1 , bone sialoprotein 1 , 44 kDa bone phosphoprotein , calcium oxalate crystal growth inhibitor protein , early T-lymphocyte activation 1 protein , minopontin , osteopontin-like protein , Sialoprotein (osteopontin) , bone sialoprotein I , osteopontin-K , OC-1 , secreted phosphoprotein-1 , SPPase1 , sphingosine-1-phosphatase 1 , hSPP1 , hSPPase1 , spp1

432385 Danio rerio
6696 Homo sapiens
478471 Canis lupus familiaris
397087 Sus scrofa
20750 Mus musculus
25353 Rattus norvegicus
281499 Bos taurus
100008982 Oryctolagus cuniculus
395210 Gallus gallus
443058 Ovis aries
100860805 Capra hircus
81535 Mus musculus
81537 Homo sapiens
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