Osteopontin antibody (C-Term)

Catalog No. ABIN2774904

Cited in 3+ publications. The Rabbit Polyclonal anti-Osteopontin antibody (ABIN2774904) specifically detects Osteopontin in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Dog, Cow, Guinea Pig, Sheep and Goat samples.

Quick Overview for Osteopontin antibody (C-Term) (ABIN2774904)

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Sheep, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Osteopontin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Osteopontin Antibody

Binding Specificity: C-Term

Purpose: SPP1 Antibody - C-terminal region

Sequence: DSQELSKVSR EFHSHEFHSH EDMLVVDPKS KEEDKHLKFR ISHELDSASS

Predicted Reactivity: Cow: 86%, Dog: 79%, Goat: 79%, Guinea Pig: 86%, Human: 100%, Mouse: 86%, Rat: 79%, Sheep: 79%

Characteristics: This is a rabbit polyclonal antibody against SPP1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SPP1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8111241

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Osteopontin antibody (ABIN2774904)

Baltatu, Vizureanu, Sandu, Solcan, Hritcu, Spataru: "Research Progress of Titanium-Based Alloys for Medical Devices." in: Biomedicines, Vol. 11, Issue 11, (2023) (PubMed).

Spataru, Cojocaru, Sandu, Solcan, Duceac, Baltatu, Voiculescu, Geanta, Vizureanu: "Assessment of the Effects of Si Addition to a New TiMoZrTa System." in: Materials (Basel, Switzerland), Vol. 14, Issue 24, (2021) (PubMed).

Baltatu, Spataru, Verestiuc, Balan, Solcan, Sandu, Geanta, Voiculescu, Vizureanu: "Design, Synthesis, and Preliminary Evaluation for Ti-Mo-Zr-Ta-Si Alloys for Potential Implant Applications." in: Materials (Basel, Switzerland), Vol. 14, Issue 22, (2021) (PubMed).

Target Details for Osteopontin

Target: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Alternative Name: SPP1

Background:

Background Information: The SPP1 gene encodes an acidic matrix protein, mainly expressed in mineralized tissues, kidney and atherosclerotic vessels. This protein also contributes to several steps in the process of prostate carcinogenesis and metastasis.

Gene Name: Secreted phosphoprotein 1

Alternative Symbols: OPN, BNSP, BSPI, ETA-1

Protein Name: Osteopontin

Molecular Weight: 33kDa

Gene ID: 6696

NCBI Accession: NP_000573

UniProt: P10451

Pathways: Regulation of Cell Size