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Characteristics
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Protein A/G MagBeads (Cat. No. L00277, 2 ml 25% slurry) are average 40 ?m in size, superparamagnetic beads covalently coated with Protein A/G. The beads are supplied as 25% slurry in phosphate buffer saline containing 20% ethanol. The Protein A/G MagBeads are ready-to-use for immunoprecipitation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses and also binds somewhat to IgA, IgE, IgM and, to a lesser extent, IgD. Therefore, it has a much broader binding range than either Protein A or Protein G individually. Unlike non-recombinant Protein G, Protein A/G does not bind serum albumin because the gene sequence coding for the albumin-binding site has been eliminated. Protein A/G is effective for mouse monoclonal antibody purification from IgG subclasses because Protein A/G binds all mouse IgG subclasses but does not bind murine IgA, IgM or serum albumin.
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