Rabbit anti-Human IgG (F(ab')2 Region) Antibody

Antigen: IgG

Binding Site: F(ab')2 Region

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChiP), Neutralization (Neut)

Catalog no.: ABIN101627

Additional Information

Immunogen: Anti-Human IgG fragment was produced in rabbit by repeated immunization with human IgG fragment.

Format: Liquid

Isotype: IgG

Description: Anti-Human IgG F(ab')2 antibody detects human IgG F(ab')2 fragments.

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Specificity: IgG F(ab')2

Application Details

Application Notes: Anti-Human IgG F(ab')2 antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:350,000
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Western Blot Dilution: 1:3,000 - 1:15,000

Concentration: 2.2 mg/mL

Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.

Purification: Sterile filtered

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium azide

Storage: Expiration date is one (1) year from date of opening. None. Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. 

Research Area: Immunology, Secondary Antibodies

Restrictions: For Research Use only

Publications

Titus JA, Haugland R, Sharrow SO et al.: "Texas Red, a hydrophilic, red-emitting fluorophore for use with fluorescein in dual parameter flow microfluorometric and fluorescence microscopic studies." in: J Immunol Methods, Vol. 50, Issue 2, pp. 193-204, 1982 (PubMed).