Goat anti-Rabbit Immunoglobulin G (IgG) (H&L) Antibody (HRP)

Antigen: Immunoglobulin G (IgG)

Epitope: H&L

Clonality: Polyclonal

Host: Goat

Reactivity: Rabbit

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN101990

Additional Information

Alternative name: Immunoglobulin G (H+L)

Immunogen: Rabbit IgG, whole molecule

Format: Lyophilized

Description: Peroxidase Conjugated Affinity Purified anti-Rabbit IgG (H&L) [Goat]

Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.

Application Details

Application Notes: All assays should be optimized by the user. Recommended dilutions (if any) may be listed below. ELISA 1:170,000 WB 1:2,000-1:20,000 IHC 1:1,000-1:5,000

Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free. Preservative: 0.01% (w/v) Gentamicin Sulfate. (Do not add Sodium Azide) Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage, restore with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Restrictions: For Research Use only

Publications

Publications: Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).