Rabbit anti-Sheep (Ovine) Immunoglobulin G (IgG) Antibody (HRP)

Antigen: Immunoglobulin G (IgG)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Sheep (Ovine)

Conjugate: HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN102255

Additional Information

Alternative name: Immunoglobulin G

Immunogen: Sheep IgG whole molecule

Format: Lyophilized

Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG and Sheep Serum.

Application Details

Application Notes: All assays ahould be optimized by the user. Recommended dilutions may be listed below: ELISA 1:100000, WB 1:1000-1:1599, IHC, 1:500-1:2500

Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free. Preservative: 0.01% (w/v) Gentamicin Sulfate. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Research Area: Secondary Antibodies

Restrictions: For Research Use only

Publications

Publications: Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).