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Goat anti-Human IgM (Heavy & Light Chain) Antibody - Preadsorbed

ELISA, IHC, WB Polyclonal IgG unconjugated
Catalog No. ABIN102624
  • Target See all IgM products
    IgM
    Binding Specificity
    • 141
    • 118
    • 87
    • 40
    • 17
    • 8
    • 7
    • 1
    • 1
    Heavy & Light Chain
    Reactivity
    • 188
    • 130
    • 65
    • 39
    • 32
    • 26
    • 22
    • 21
    • 16
    • 15
    • 15
    • 14
    • 13
    • 13
    • 12
    • 4
    • 2
    Human
    Host
    • 320
    • 188
    • 73
    • 15
    • 14
    • 5
    • 4
    • 1
    • 1
    Goat
    Clonality
    • 495
    • 78
    Polyclonal
    Application
    • 332
    • 249
    • 238
    • 208
    • 143
    • 139
    • 64
    • 63
    • 47
    • 47
    • 46
    • 42
    • 38
    • 29
    • 20
    • 18
    • 13
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificity
    IgM µ chain
    Cross-Reactivity
    Human
    Characteristics
    Anti-Mouse IgG3 heavy chain antibody generated in rabbit detects specifically Mouse IgG3 heavy chain. This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Human IgM whole molecule

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Human IgM mu heavy chain is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

    ELISA Dilution: 1:10,000 - 1:50,000

    Western Blot Dilution: 1:1,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Expiry Date
    12 months
  • Yang, Jacobs, McCallen, Moore, Huckaby, Edelstein, Lai: "Analysis of Pre-existing IgG and IgM Antibodies against Polyethylene Glycol (PEG) in the General Population." in: Analytical chemistry, Vol. 88, Issue 23, pp. 11804-11812, (2018) (PubMed).

  • Target
    IgM
    Alternative Name
    IgM mu (IgM Products)
    Target Type
    Antibody
    Background

    Synonyms: goat anti-Human IgM mu chain Antibody, Gt-a-Human IgM, Human IgM Antibody in Goat, Human Secondary Antibody

    Background: Anti-Human IgM (mu heavy chain) generated in goat detects specifically Human IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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