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Goat anti-Human Immunoglobulin M (IgM) Antibody (Alkaline Phosphatase)

Antigen

Immunoglobulin M (IgM)

Clonality Polyclonal
Host
Reactivity
Alternatives

Human

Conjugate
Alternatives Alkaline Phosphatase (AP)
Application
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Catalog no. ABIN102625
Quantity 1mg  (1.0 mg/ml (by UV absorbance at 280 ...)  (Variants)
Price 170.00 $   Plus shipping costs $35.00
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Additional Information

Alternative name Immunoglobulin M
Immunogen Human IgM u mu chain
Format Liquid (sterile filtered)

Application Details

Application Notes Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgM in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Buffer Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0.
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Restrictions For Research Use only

Alternatives

Alternatives for antigen "Immunoglobulin M (IgM)", type "Secondary Antibodies"
Hosts Goat (194), Rabbit (129), Mouse (35), Rat (14), Chicken (1)
Reactivities Human (112), Mouse (Murine) (100), Rat (Rattus) (51), Monkey (19), Chicken (18), Ferret (13), Sheep (Ovine) (8), Cat (Feline) (7), Cow (Bovine) (7), Dog (Canine) (7), Horse (Equine) (7), Rabbit (7), Goat (6), Pig (Porcine) (6), Hamster (4), Syrian Hamster (2), Donkey (1), Guinea Pig (1), Turkey (1)
Conjugates FITC (67), HRP (64), Biotin (45), Alkaline Phosphatase (AP) (39), Texas Red (TR) (24), TRITC (20), Rhodamine (14), PE (8), DyLight 488 (6), Peroxidase (POD) (5), APC (4), Colloidal Gold (15 OD) (2), Colloidal Gold (50 OD) (2), RPE (2), SPRD (2), Cy5 (1), DyLight 405 (1), DyLight 594 (1), DyLight 680 (1), DyLight 800 (1), PE,Cy7 (1)
Epitopes Heavy chain (100), H&L (18), Fc5m (7), Whole (7), Fc5u (3), Fc- (2), Fc5u specific (1), Fcu (1)