Rabbit anti-Human Immunoglobulin M (IgM) Antibody (Peroxidase)

Antigen: Immunoglobulin M (IgM)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Peroxidase (POD)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN102645

Additional Information

Alternative name: Immunoglobulin M

Immunogen: Human IgM Fc5 u fragment

Format: Lyophilized

Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgM and Human Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.

Application Details

Application Notes: Recommended Dilutions: This product has been assayed against 1.0 ug of Human IgM in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:8,000 to 1:30,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Concentration: 1.5 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free. Preservative: 0.01% (w/v) Gentamicin Sulfate. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage mix product with 50% glycerol and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Restrictions: For Research Use only

Publications

Publications: Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).