BrdU antibody (PE)

Catalog No. ABIN1303172

Product Details anti-BrdU Antibody (PE)

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. It cross-reacts with EdU.

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Bromodeoxyuridine conjugated to BSA

Clone: Bu20a

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-BrdU antibody (PE) (ABIN1303172)

Yatera, Hsieh, Hogg, Tranfield, Suzuki, Shih, Behzad, Vincent, van Eeden: "Particulate matter air pollution exposure promotes recruitment of monocytes into atherosclerotic plaques." in: American journal of physiology. Heart and circulatory physiology, Vol. 294, Issue 2, pp. H944-53, (2008) (PubMed).

ODriscoll, Jeggo: "CsA can induce DNA double-strand breaks: implications for BMT regimens particularly for individuals with defective DNA repair." in: Bone marrow transplantation, Vol. 41, Issue 11, pp. 983-9, (2008) (PubMed).

Lagares, Li, Zhou, Avendaño: "Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 30, pp. 7939-53, (2007) (PubMed).

Pajalunga, Mazzola, Salzano, Biferi, De Luca, Crescenzi: "Critical requirement for cell cycle inhibitors in sustaining nonproliferative states." in: The Journal of cell biology, Vol. 176, Issue 6, pp. 807-18, (2007) (PubMed).

Martín, Surur, García, Corrons, Badrán: "Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver." in: Biocell : official journal of the Sociedades Latinoamericanas de Microscopía Electronica ... et. al, Vol. 29, Issue 1, pp. 33-8, (2005) (PubMed).

Shih, Whalen, Goto, Hogg, van Eeden: "Flow cytometric method for enumeration and characterization of newly released polymorphonuclear leukocytes from the bone marrow using 5'-bromo-2'-deoxyuridine." in: American journal of physiology. Cell physiology, Vol. 289, Issue 3, pp. C757-65, (2005) (PubMed).

Muskhelishvili, Latendresse, Kodell, Henderson: "Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67(MIB-5) immunohistochemistry and in situ hybridization for histone mRNA." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 51, Issue 12, pp. 1681-8, (2003) (PubMed).

Magaud, Sargent, Clarke, Ffrench, Rimokh, Mason: "Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 37, Issue 10, pp. 1517-27, (1989) (PubMed).

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: 5-bromodeoxyuridine (BrdU) (BrdU Products)

Target Type: Chemical

Background: Bromodexyuridine (BrdU) is a thymidine analog, which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.,5-bromodeoxyuridine