Bromodeoxyuridine (BrDU) antibody

Antigen: Bromodeoxyuridine (BrDU)

Clonality: Monoclonal (Bu20a)

Host: Mouse

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN1043026

Additional Information

Alternative name: 5-bromodeoxyuridine (BrdU)

Immunogen: Bromodeoxyuridine conjugated to BSA

Isotype: IgG1

Clone: Bu20a

Specificity: The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining.

Comments:

Small Organic Molecules

Application Details

Buffer: Phosphate buffered saline (PBS) and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
Do not use after expiration date stamped on vial label.
Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Restrictions: For Research Use only

Publications

Product: Magaud, Sargent, Clarke et al.: "Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 37, Issue 10, pp. 1517-27, 1989 (PubMed).

Muskhelishvili, Latendresse, Kodell et al.: "Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67(MIB-5) immunohistochemistry and in situ hybridization for histone mRNA." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 51, Issue 12, pp. 1681-8, 2003 (PubMed).

Shih, Whalen, Goto et al.: "Flow cytometric method for enumeration and characterization of newly released polymorphonuclear leukocytes from the bone marrow using 5'-bromo-2'-deoxyuridine." in: American journal of physiology. Cell physiology, Vol. 289, Issue 3, pp. C757-65, 2005 (PubMed).

Martín, Surur, García et al.: "Comparative study of DNA synthesis and nucleolar organizer regions of sinusoid littoral cells in mouse regenerating liver." in: Biocell : official journal of the Sociedades Latinoamericanas de Microscopía Electronica ... et. al, Vol. 29, Issue 1, pp. 33-8, 2005 (PubMed).

Pajalunga, Mazzola, Salzano et al.: "Critical requirement for cell cycle inhibitors in sustaining nonproliferative states." in: The Journal of cell biology, Vol. 176, Issue 6, pp. 807-18, 2007 (PubMed).

Lagares, Li, Zhou et al.: "Primary sensory neuron addition in the adult rat trigeminal ganglion: evidence for neural crest glio-neuronal precursor maturation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 30, pp. 7939-53, 2007 (PubMed).

Yatera, Hsieh, Hogg et al.: "Particulate matter air pollution exposure promotes recruitment of monocytes into atherosclerotic plaques." in: American journal of physiology. Heart and circulatory physiology, Vol. 294, Issue 2, pp. H944-53, 2008 (PubMed).

ODriscoll, Jeggo: "CsA can induce DNA double-strand breaks: implications for BMT regimens particularly for individuals with defective DNA repair." in: Bone marrow transplantation, Vol. 41, Issue 11, pp. 983-9, 2008 (PubMed).