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CD5 Molecule (CD5) antibody

Details for Product No. ABIN1043027
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Antigen
Synonyms T1, LEU1, CD5
Reactivity
Human
(315), (146), (77), (39), (28), (25), (19), (12), (9), (1), (1)
Host
Mouse
(356), (105), (88)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(92), (62), (60), (26), (13), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS), Western Blotting (WB)
(396), (104), (86), (80), (80), (60), (40), (35), (24), (16), (13), (11), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN1043027
290.40 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Human acute lymphoblastic leukemia (ALL) T cells
Clone L17F12
Isotype IgG2a
Specificity The mouse monoclonal antibody L17F12 reacts with CD5, a 67 kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name CD5
Background CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
Gene ID 921
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Application Notes Working concentrations should be determined by the investigator.I t is recommended that the user titrates the reagent for use in the particular testing system.

Working concentrations should be determined by the investigator.
Comment

CD and Related Antigens (Human)

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Product cited in: Shuster, Falletta, Pullen et al.: "Prognostic factors in childhood T-cell acute lymphoblastic leukemia: a Pediatric Oncology Group study." in: Blood, Vol. 75, Issue 1, pp. 166-73, 1990 (PubMed).

Engleman, Warnke, Fox et al.: "Studies of a human T lymphocyte antigen recognized by a monoclonal antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 78, Issue 3, pp. 1791-5, 1981 (PubMed).

McAlister, Davis, Pfuhl et al.: "NMR analysis of the N-terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments." in: Protein engineering, Vol. 11, Issue 10, pp. 847-53, 1999 (PubMed).

Gong, Lagoo, Peters et al.: "Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma." in: American journal of clinical pathology, Vol. 116, Issue 6, pp. 893-7, 2001 (PubMed).

Dunphy, Tang: "The value of CD64 expression in distinguishing acute myeloid leukemia with monocytic differentiation from other subtypes of acute myeloid leukemia: a flow cytometric analysis of 64 cases." in: Archives of pathology & laboratory medicine, Vol. 131, Issue 5, pp. 748-54, 2007 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN1043027
290.40 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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