Epstein-Barr Virus Induced 3 (EBI3) antibody

Antigen: Epstein-Barr Virus Induced 3 (EBI3)

Protein Type: Recombinant

Clonality: Monoclonal (V1.4C4.22)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Please enquire

Catalog no.: ABIN1043814

Additional Information

Alternative name: EBI-3

Gene ID: 50498

NCBI Acession: NP_056581.1

UniProt: O35228

Immunogen: Anti-EBI-3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.
Immunogen Type: Recombinant Protein

Format: Liquid

Isotype: IgG2b

Clone: V1.4C4.22

Description: Anti-Mouse EBI-3 antibody has been tested for use in ELISA , IP, and Neutralization. Specific conditions for reactivity should be optimized by the end user.

Synonyms: Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein.

Characteristics: The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.

Specificity: Anti-Mouse EBI-3 (MOUSE) Monoclonal Antibody was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse EBI3 protein. A BLAST analysis was used to suggest cross-reactivity with EBI3 from mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with EBI-3 from other sources has not been determined.

Comments:

Gene Name: EBI3

Sterility: sterile filtered

Application Details

Application Notes: ELISA Dilution: 1:10,000.
Immunoprecipitation Dilution: 1µg/mL.
Western Blot Dilution: 1:1000.

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Storage: 4°C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Images

ELISA - EBI-3 Antibody. Supernatant from 293T cells transfected with a vector containing murine IL-35 was added to an ELISA plate coated with V1.4H6.29 at 2ug/ml in PBS. mEBI3 was detected with biotinylated V1.4C4.22.

Publications

Product: Collison, Workman, Kuo et al.: "The inhibitory cytokine IL-35 contributes to regulatory T-cell function." in: Nature, Vol. 450, Issue 7169, pp. 566-9, 2007 (PubMed).