Interleukin 17F (IL17F) antibody (HRP)

Antigen: Interleukin 17F (IL17F)

Synonyms: ML1, ML-1, IL-17F, C87042, MGC114402, IL17F, IL17, IL-17, IL17A, ChIL-17

Protein Type: Recombinant

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Rat (Rattus)

Conjugate: HRP

Application: ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Catalog no.: ABIN1043827

Additional Information

Alternative name: IL-17F

Gene ID: 257630

NCBI Acession: NP_665855.2

UniProt: Q7TNI7

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL17-F protein.
Immunogen Type: Recombinant Protein

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL

Isotype: IgG

Description: This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 17.9 kDa in size corresponding to mouse IL17-F protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Synonyms: IL-17F, Interleukin-17F, ML1, ML-1, IL17F, and IL24.

Characteristics: IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.

Specificity: This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse IL17-F in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.

Comments:

Gene Name: Il17f

Application Details

Application Notes: ELISA Dilution: 1:10,000-1:50,000.
Immunohistochemistry Dilution: 1:500-1:2,500.
Western Blot Dilution: 1:1,000-1:5,000.

Handling Advice: Do NOT add Sodium Azide!

Concentration: 1.0 mg/mL

Purification: purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only

Publications

Product: Gomez-Rodriguez, Sahu, Handon et al.: "Differential expression of interleukin-17A and -17F is coupled to T cell receptor signaling via inducible T cell kinase." in: Immunity, Vol. 31, Issue 4, pp. 587-97, 2009 (PubMed).