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Interleukin 17F (IL17F) antibody (HRP)
|Synonyms||ML1, ML-1, IL-17F, C87042, MGC114402, IL17F, IL17, IL-17, IL17A, ChIL-17|
Mouse (Murine), Rat (Rattus)
Alternatives ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
|1 reference available|
|Quantity||100 µg (1.0 mg/mL) (Variants)|
|Price||345.40 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL17-F protein.
Immunogen Type: Recombinant Protein
|Reconstitution||Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL|
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 17.9 kDa in size corresponding to mouse IL17-F protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Synonyms: IL-17F, Interleukin-17F, ML1, ML-1, IL17F, and IL24.
|Characteristics||IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.|
|Specificity||This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse IL17-F in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.|
Gene Name: Il17f
ELISA Dilution: 1:10,000-1:50,000.
Immunohistochemistry Dilution: 1:500-1:2,500.
Western Blot Dilution: 1:1,000-1:5,000.
|Handling Advice||Do NOT add Sodium Azide!|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!|
|Storage Comment||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Expiry Date||Expiration date is one (1) year from date of opening.|
|Restrictions||For Research Use only|
Gomez-Rodriguez, Sahu, Handon et al.: "Differential expression of interleukin-17A and -17F is coupled to T cell receptor signaling via inducible T cell kinase." in: Immunity, Vol. 31, Issue 4, pp. 587-97, 2009 (PubMed).