Exosome Component 1 (EXOSC1) antibody

Antigen: Exosome Component 1 (EXOSC1)

Synonyms: p13, CSL4, SKI4, Csl4p, Ski4p, hCsl4p, CGI-108, RP11-452K12.9, AI447561, 2610035C18Rik, 2610104C07Rik, 2610312F07Rik, MGC86284

Clonality: Monoclonal (BM3)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN105211

Additional Information

Alternative name: p13

Immunogen: A BALB/c mouse was immunized with nuclei from Pokeweed mitogen stimulated human peripheral blood lymphocytes. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse cells using conventional hybridoma technology.

Format: Liquid (sterile filtered)

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: BM3

Description: P13 is a specific marker for human myeloid cells. Induction studies using HL-60 cells show that p13 is expressed during the early phases of myeloid differentiation. The mAb anti-p13 recognizes a nuclear antigen expressed in human granulocytes (98%) and monocytes (80%) residing in lymphoid and non-lymphoid tissues. BM3 is an early marker of myeloid differentiation. Immunoprecipitation experiments using S 35 methionine labeled human myeloid leukemia cells show that BM3 identifies a 13,000 Dalton protein.

Specificity: This protein A purified mouse monoclonal antibody reacts specifically with p13 in human granulocytes and monocytes residing in lymphoid and non-lymphoid tissues The antibody recognizes a 13 kDa band corresponding to p13. Cross reactivity from other sources has not been determined.

Application Details

Application Notes: Recommended Dilutions: For immunohistochemistry dilute the antibody 1:100. Researchers should determine optimal titers for other applications. Note: This antibody is suitable for flow cytometry and frozen section immunohistochemistry. The antibody does not stain tissue after paraffin treatment.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Restrictions: For Research Use only