p29 ING4 (AA 164-175,Isoform 1) antibody

Antigen: p29 ING4

Binding Site: AA 164-175,Isoform 1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN105223

Additional Information

Gene ID: 51147

UniProt: Q9UNL4

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 164-175 of Human p29 ING4 protein (Inhibitor of growth family, member 4). This sequence only shows homology to for ING4.

Format: Liquid

Isotype: IgG

Description: P29 ING4 is a tumor suppressor protein similar to ING1 that may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. p29 ING4 has been shown to suppress brain tumor angio-genesis through transcriptional repression of RelA/ NFKB3 target genes when complexed with RelA. p29 ING4 may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. p29 ING4 shows a nuclear localization and interacts with EP300, TP53, RelA, inhibits cell growth, and induces apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) that encodes the longest isoform.

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Application Details

Application Notes: This affinity-purified antibody has been tested for use in ELISA against the immunizing peptide. Specific conditions for western blotting reactivity should be optimized by the end user. Expect a band at approximately 29 kDa in size corresponding to ING4 isoform 1 by western blotting in the appropriate cell lysate or extract.
ELISA Dilution: 1:3,000 - 1:12,000
Western Blot Dilution: 1:500 - 1:3,000

Concentration: 1.28 mg/mL

Purification: Sterile filtered

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium azide

Storage: Expiration date is one (1) year from date of opening. None. Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Restrictions: For Research Use only

Images

Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot in over expressed cell lysates.  This antibody was used at 1.0 µg/ml to detect ING4 expression in control (-) and transformed U2OS and HeLa cell lysates.  A predominant band corresponding to p29 ING4 is only seen in lysates from transformed cells.  Personnel Communication, Motoko Unoki, NCI, NIH.

Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot in over expressed cell lysates. This antibody was used at 1.0 ug/ml to detect ING4 expression in control (-) and transformed U2OS and Hcell lysates. A predominant band corresponding to p29 ING4 is only seen in lysates from transformed cells. Personnel Communication, Motoko Unoki, NCI, NIH.

Publications

Unoki, Shen, Zheng et al.: "Novel splice variants of ING4 and their possible roles in the regulation of cell growth and motility." in: The Journal of biological chemistry, Vol. 281, Issue 45, pp. 34677-86, 2006 (PubMed).

Colla, Tagliaferri, Morandi et al.: "The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesi" in: Blood, Vol. 110, Issue 13, pp. 4464-75, 2007 (PubMed).