RFX5 (AA 320-494) antibody

Antigen: RFX5

Epitope: AA 320-494

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine), Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN106544

Additional Information

Alternative name: RFX5, (AA 320-494)

Immunogen: RFX5 peptide corresponding to a region at amino acids 320 to 494 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Format: Liquid (sterile filtered)

Specificity: This product was prepared from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography. Anti-RFX5 (aa 320 to 494) may react with unknown minor bands at 65 kDa and 80 kDa.

Application Details

Application Notes: Recommended Dilutions: This product was assayed by immunoblot and found to be reactive against RFX5 (aa 320 to494) from a variety of fibroblast and B-cell lysates at a dilution of1:1,000 followed by reaction with Peroxidase conjugated AffinityPurified anti-Rabbit IgG [H&L] (Goat) code ABIN101990. Anti-RFX5 (aa 320 to 494) detects a 75 kDa band by immunoblot forhuman RFX5. This product was also tested in a gel supershiftassay and found to be reactive against RFX5 complexes using0.5 to 1.0 u l per assay. Optimal titers for other applications should be determined by the researcher. Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays.

Concentration: 0.68 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is six (6) months from date of opening product.

Restrictions: For Research Use only

Images

Anti-RFX5 (aa 320 to 494) is shown to detect RFX5 present in Raji B cell nuclear extract lysates. Detection occurs using a 1:2,500 dilution of antibody followed by 1:5,000 dilution of HRP Goat-a-Rabbit IgG with visualization via ECL. Film exposure approximately 1

Publications

Publications: Xu, Sengupta, Seto et al.: "Regulatory factor for X-box family proteins differentially interact with histone deacetylases to repress collagen alpha2(I) gene (COL1A2) expression." in: The Journal of biological chemistry, Vol. 281, Issue 14, pp. 9260-70, 2006 (PubMed).