NEDD9 antibody (AA 82-398)

Catalog No. ABIN108552

Cited in 1 publication. The Mouse Monoclonal anti-NEDD9 antibody (Clone 14A11) (ABIN108552) specifically detects NEDD9 in IF, IP and ICC. The antibody is reactive with Human samples.

Quick Overview for NEDD9 antibody (AA 82-398) (ABIN108552)

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEDD9 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 14A11

Product Details anti-NEDD9 Antibody

Binding Specificity: AA 82-398

Specificity: Specific for human HEF1 localized in mitotic cells in the spindle and the centrosome. Does not recognize p130 Cas. Has not been tested on Sin1

Purification: Protein G

Immunogen: Fusion protein, corresponding to amino acids 82-398 of Human HEF1.

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try,IP: Use a concentration of 1 μg/mL. Use at a concentration of 1 μg/200 μg whole cell lysate.IF: 1:100

Comment:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody containing PBS + 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

References for anti-NEDD9 antibody (ABIN108552)

ONeill, Golemis: "Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics." in: Molecular and cellular biology, Vol. 21, Issue 15, pp. 5094-108, (2001) (PubMed).

Target Details for NEDD9

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Alternative Name: HEF1/NEDD-9

Background: Synonyms:
CAS L antibody,Cas like docking antibody,Cas scaffolding protein family member 2 antibody,CAS-Lantibody,CAS2 antibody,CASL antibody,CASL_HUMAN antibody,CASS2 antibody,Crk associated substrate relatedprotein antibody,CRK-associated substrate-related protein antibody,dJ49G10.2 (Enhancer of Filamentation 1 (HEF1))antibody,dJ49G10.2 antibody,dJ761I2.1 (enhancer of filamentation (HEF1)) antibody,dJ761I2.1 antibody,Enhancerof filamentation 1 antibody,Enhancer of filamentation 1 p55 antibody,HEF 1 antibody,HEF1 antibody,NEDD 9antibody,NEDD-9 antibody,NEDD9 antibody,NEDD9 protein antibody,Neural cell expressed developmentally downregulated 9 antibody,Neural precursor cell expressed developmentally down regulated 9 antibody,Neural precursorcell expressed developmentally down-regulated protein 9 antibody,NY REN 12 antigen antibody,p105antibody,Protein NEDD9 antibody,Renal carcinoma antigen NY-REN-12 antibody

OMIM: 602265