NEDD9 antibody (AA 82-398)

Catalog No. ABIN108573

There are 3+ publications for this product available. The Mouse Monoclonal anti-NEDD9 antibody is suitable to detect NEDD9 in samples from Human, Rat and Mouse. It has been validated for WB, IF, IP, FACS, ICC and IHC (fro).

Quick Overview for NEDD9 antibody (AA 82-398) (ABIN108573)

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEDD9 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2G9

Product Details anti-NEDD9 Antibody

Binding Specificity: AA 82-398

Specificity: Does not recognize p130 Cas. Not tested on Sin1. This antibody mostly detects HEF1 localized at the focal adhesion sites

Purification: Protein G

Immunogen: Fusion protein, corresponding to amino acids 82-398 of Human HEF1.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try, WB: 1/2000 - 1/6000. Predicted molecular weight: 93 kDa. IP: 1/1000. ICC/IF: Use at an assay dependent dilution. PubMed: 19376971 IHC-Fr: Use at an assay dependent dilution. PubMed: 19464348 Flow Cyt: Use 1 μg for 106 cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody containing PBS 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

References for anti-NEDD9 antibody (ABIN108573)

Tomkiewicz, Herry, Bui, Métayer, Bourdeloux, Barouki, Coumoul: "The aryl hydrocarbon receptor regulates focal adhesion sites through a non-genomic FAK/Src pathway." in: Oncogene, Vol. 32, Issue 14, pp. 1811-20, (2013) (PubMed).

Zhong, Baquiran, Bonakdar, Lees, Ching, Pugacheva, Fabry, ONeill: "NEDD9 stabilizes focal adhesions, increases binding to the extra-cellular matrix and differentially effects 2D versus 3D cell migration." in: PLoS ONE, Vol. 7, Issue 4, pp. e35058, (2012) (PubMed).

ONeill, Golemis: "Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics." in: Molecular and cellular biology, Vol. 21, Issue 15, pp. 5094-108, (2001) (PubMed).

Target Details for NEDD9

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Alternative Name: HEF1

Background: Synonyms:
CAS L antibody,Cas like docking antibody,CASL antibody,Crk associated substrate related proteinantibody,dJ49G10.2 antibody,dJ761I2.1 antibody,Enhancer of filamentation 1 antibody,HEF 1 antibody,NEDD 9antibody,NEDD9 antibody,NEDD9 protein antibody,Neural cell expressed developmentally down regulated 9antibody,Neural precursor cell expressed developmentally down regulated 9 antibody,NY REN 12 antigenantibody,p105 antibody,Protein NEDD9 antibody

Gene ID: 4739, 18003, 291044

OMIM: 602265

UniProt: O35177