Transportin 1 antibody

Catalog No. ABIN108619

The Mouse Monoclonal anti-Transportin 1 antibody has been validated for WB, ELISA, IP, ICC, FACS, IHC (p) and IF. It is suitable to detect Transportin 1 in samples from Human, Mouse, Monkey and Rabbit. There are 6+ publications available.

Quick Overview for Transportin 1 antibody (ABIN108619)

Target: Transportin 1 (TNPO1)

Reactivity: Human, Mouse, Monkey, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transportin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Clone: D45

Product Details anti-Transportin 1 Antibody

Purification: Protein A

Immunogen: Recombinant his tagged transportin1 (Human)

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try,Flow Cyt: Use 1 μg for 106 cells.ICC/IF: Use a concentration of 5 μg/mL.IHC-P: Use a concentration of 1 μg/mL. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.WB: 1:800. Detects a band of approximately 93 kDa (predicted molecular weight: 107 kDa)

Comment:

Myeloma, fusion partners: SP2/0

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS + 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Transportin 1 antibody (ABIN108619)

Yu, Boyce, Wands, Bond, Bertozzi, Kohler: "Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 13, pp. 4834-9, (2012) (PubMed).

Brelstaff, Lashley, Holton, Lees, Rossor, Bandopadhyay, Revesz: "Transportin1: a marker of FTLD-FUS." in: Acta neuropathologica, Vol. 122, Issue 5, pp. 591-600, (2011) (PubMed).

Gu, Tsuji, Jarboui, Yeo, Sheehy, Hall, Gautier: "Intermolecular masking of the HIV-1 Rev NLS by the cellular protein HIC: novel insights into the regulation of Rev nuclear import." in: Retrovirology, Vol. 8, pp. 17, (2011) (PubMed).

Chen, Shao, Cobos, Wang, Gao: "4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone [corrected] induces CRM1-dependent p53 nuclear accumulation in human bronchial epithelial cells." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 116, Issue 1, pp. 206-15, (2010) (PubMed).

Janiszewska, De Vito, Le Bitoux, Fusco, Stamenkovic: "Transportin regulates nuclear import of CD44." in: The Journal of biological chemistry, Vol. 285, Issue 40, pp. 30548-57, (2010) (PubMed).

Favre, Camps, Arod, Chabert, Rommel, Pasquali: "Chemokine receptor CCR2 undergoes transportin1-dependent nuclear translocation." in: Proteomics, Vol. 8, Issue 21, pp. 4560-76, (2008) (PubMed).

Target Details for Transportin 1

Target: Transportin 1 (TNPO1)

Alternative Name: Transportin 1 

Background: Synonyms:
Importin beta 2 antibody,IPO 2 antibody,IPO2 antibody,Karyopherin (importin) beta 2antibody,Karyopherin beta 2 antibody,KPN B2 antibody,KPNB 2 antibody,KPNB2 antibody,M9 interacting proteinantibody,M9 region interacting protein antibody,M9 region interaction protein antibody,M9-interacting proteinantibody,MIP 1 antibody,MIP antibody,MIP1 antibody,TNPO 1 antibody,TNPO1 antibody,Transportinantibody,Transportin-1 antibody,Transportin1 antibody,TRN antibody

OMIM: 602901

Pathways: PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cellular Glucan Metabolic Process, Protein targeting to Nucleus, CXCR4-mediated Signaling Events