CEACAM1/3/5/8 (CEACAM1/3/5/8) antibody

Antigen: CEACAM1/3/5/8 (CEACAM1/3/5/8)

Clonality: Monoclonal (TET2)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Catalog no.: ABIN108728

Additional Information

Alternative name: CEACAM1, 5, 6, 8 (CD66abce)

Immunogen: immunisation with extracted protein of CEACAM5

Format: Purified

Isotype: IgG2b  (Matching secondary antibodies)

Clone: TET2

Description: CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). It consists of seven CEACAM (CEACAM1, CEACAM3- CEACAM8) and 11 pregnancy- specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constantlike (IgC) domains (Fig. 2). CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor (Fig. 2). CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (2). The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (1).

Specificity: Anti-human CEACAM1,5,6,8 (CD66a,b,c,e)

Application Details

Application Notes: Flow cytometry: 1 ug/106 cells. ELISA: 1:200 - 1:400. CELISA: 1:200. Western Blot: 4ug/ml. Immunohistology: 1-2 ug/106 cells (on cryosections)

Concentration: 1mg/ml

Purification: Protein G

Buffer: phosphate buffered saline, pH 7.2

Storage: Short term: 2°C - 8°C. Long term: -20°C (avoid repeated freezing and thawing)

Research Area: Receptors, Immunology, Cancer, Receptors, Cell/Tissue Markers, Cancer

Restrictions: For Research Use only

Images

FACS analysis of BOSC23 cells using TET2. BOSC23 cells were transiently transfected with anexpression vector encoding either CEACAM1,5,6,8 (red curves) or an irrelevant protein (control transfectant). Binding ofTET2 was detected with a PE conjugated seco

Domain organization of the human CEACAM family. The CEACAM family consists of two subgroups, the CEACAM and the PSG subgroup. Old names appear below the consensus nomenclature. CEACAM family members are membrane-bound by either via a transmembrane domain

Specificity testing of TET2. BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembrane-anchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells

Publications

Publications: Grunert, AbuHarfeil, Schwarz et al.: "Two CEA and three NCA species, although distinguishable by monoclonal antibodies, have nearly identical peptide patterns." in: International journal of cancer. Journal international du cancer, Vol. 36, Issue 3, pp. 357-62, 1985 (PubMed).

Saeland, Belo, Mongera et al.: "Differential glycosylation of MUC1 and CEACAM5 between normal mucosa and tumour tissue of colon cancer patients." in: International journal of cancer. Journal international du cancer, 2011 (PubMed).