CEACAM5/6 (CEACAM5/6) antibody

Antigen: CEACAM5/6 (CEACAM5/6)

Clonality: Monoclonal (MUS)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Catalog no.: ABIN108730

Additional Information

Alternative name: CECAM5,6 (CEA, NCA, CD66ce)

Immunogen: immunisation with extracted protein of CEACAM5

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: MUS

Description: CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (1). It consists of seven CEACAM (CEACAM1, CEACAM3- CEACAM8) and 11 pregnancy-specific glycoprotein (PSG1-PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variablelike (IgV) and a varying number (0-6) of Ig constantlike (IgC) domains (Fig. 2). CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor (Fig. 2). CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (2). CEACAM6 expression is strongly up-regulated already during early stages of adenocarcinoma formation e.g. in colon (3). The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (1).

Specificity: Anti-human CEACAM1,3,4,5,6 (CD66a,c,d,e,CGM7)

Application Details

Application Notes: Flow cytometry: 1.2 ug/10^6 cells. ELISA: 1:200 - 1:400 CELISA: 1:200. Western blot: 4ug/ml. Immunohistology: 1-2 ug/10^6 cells (on cryosections)

Concentration: 1mg/ml

Purification: Protein G

Buffer: phosphate buffered saline, pH 7.2

Storage: short term: 2°C - 8°C; long term: -20°C (avoid repeated freezing and thawing)

Research Area: Receptors, Immunology, Cancer, Cell/Tissue Markers, Cancer

Restrictions: For Research Use only

Images

FACS analysis of BOSC23 cells using MUS. BOSC23 cells were transiently transfected with anexpression vector encoding either CEACAM5 (red curve) or an irrelevant protein (control transfectant). Binding of MUSwas detected with a PE conjugated secondary ant

FACS analysis of BOSC23 cells using MUS. BOSC23 cells were transiently transfected with anexpression vector encoding either CEACAM6 (red curve) or an irrelevant protein (control transfectant). Binding of MUS was detected with a PE conjugated secondary an

Specificity testing of MUS. BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 7, 8 or a recombinant transmembraneanchored PSG1 fusion protein. Recognition of CEACAM4 was tested on CHO cells st

Publications

Publications: Grunert, AbuHarfeil, Schwarz et al.: "Two CEA and three NCA species, although distinguishable by monoclonal antibodies, have nearly identical peptide patterns." in: International journal of cancer. Journal international du cancer, Vol. 36, Issue 3, pp. 357-62, 1985 (PubMed).

Schoelzel, Zimmermann, Schwarzkopf et al.: "Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas." in: The American journal of pathology, Vol. 156, Issue 2, pp. 595-605, 2000 (PubMed).