CEACAM1/5 (CEACAM1/5) antibody

Antigen: CEACAM1/5 (CEACAM1/5)

Clonality: Monoclonal (4-3-17)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Cell-ELISA (cELISA), Western Blotting (WB), ELISA

Catalog no.: ABIN108735

Additional Information

Alternative name: CEACAM1,5 (BGP, CEA, CD66ae)

Immunogen: immunisation with extracted protein of CEACAM5

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 4-3-17

Description: CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family (9). It consists of seven CEACAM (CEACAM1, CEACAM3-CEACAM8) and 11 pregnancy-specific glycoprotein (PSG1- PSG11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains (Fig. 2). CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor (Fig. 2). CEACAM molecules are differentially expressed in epithelial cells or in leucocytes (Fig 3). Over-expression of CEA/CEACAM5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker (8). CEACAM1 expression is down-regulated in many tumors indicating a tumor-suppressive function. The anti-tumor effect may be due to inhibition of tumor angiogenesis, possibly by increased secretion of anti-angiogenic molecules from the cells (4). The function of CEA family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria (9).

Specificity: Anti-human CEACAM1, 5 (BGP, CEA, CD66a,e)

Application Details

Application Notes: Flow cytometry: 1.2 ug/10^6 cells. ELISA: 1:200 - 1:400 CELISA: 1:200. Western blot: 4ug/ml. Immunohistology: 1-2 ug/10^6 cells (on cryosections)

Concentration: 1 mg/ml

Buffer: phosphate buffered saline, pH 7.2

Storage: Short term: 2°C - 8°C. long term: -20°C (avoid repeated freezing and thawing)

Research Area: Cancer

Restrictions: For Research Use only

Images

FACS analysis of BOSC23 cells using 4/3/17. BOSC23 cells were transiently transfected with anexpression vector encoding either CEACAM1 (red curve) oran irrelevant protein (control transfectant). Binding of 4/3/17was detected with a PE conjugated secondar

FACS analysis of BOSC23 cells using 4/3/17. BOSC23 cells were transiently transfected with anexpression vector encoding either CEACAM5 (red curve) or anirrelevant protein (control transfectant). Binding of 4/3/17wasdetected with a PE conjugated secondary

Specificity testing of 4/3/17. BOSC cells were transiently transfected with expression vectors containing either the cDNA of CEACAM1, 3, 5, 6, 8 or a recombinant transmembraneanchored PSG1 fusion protein. Expression of the constructs was confirmed with m

Publications

Publications: Grunert, AbuHarfeil, Schwarz et al.: "Two CEA and three NCA species, although distinguishable by monoclonal antibodies, have nearly identical peptide patterns." in: International journal of cancer. Journal international du cancer, Vol. 36, Issue 3, pp. 357-62, 1985 (PubMed).

Kleinerman DI, Troncoso P, Lin SH et al.: "Consistent expression of an epithelial cell adhesion molecule (C-CAM) during human prostate development and loss of expression in prostate cancer: implication as a tumor suppressor." in: Cancer Res, Vol. 55, Issue 6, pp. 1215-20, 1995 (PubMed).

Daniel S, Nagel G, Johnson JP et al.: "Determination of the specificities of monoclonal antibodies recognizing members of the CEA family using a panel of transfectants." in: Int J Cancer, Vol. 55, Issue 2, pp. 303-10, 1993 (PubMed).

Krop-Watorek, Laskowska, Salwa et al.: "CEA-related proteins on human urothelial cell lines of different transformation grades." in: Cancer letters, Vol. 139, Issue 1, pp. 15-22, 1999 (PubMed).

Schoelzel, Zimmermann, Schwarzkopf et al.: "Carcinoembryonic antigen family members CEACAM6 and CEACAM7 are differentially expressed in normal tissues and oppositely deregulated in hyperplastic colorectal polyps and early adenomas." in: The American journal of pathology, Vol. 156, Issue 2, pp. 595-605, 2000 (PubMed).

Muenzner P, Naumann M, Meyer TF et al.: "Pathogenic Neisseria trigger expression of their carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1, previously CD66a) receptor on primary endothelial cells by activating the immediate early response transcription factor, nuclear facto" in: J Biol Chem, Vol. 276, Issue 26, pp. 24331-40, 2001 (PubMed).

Muenzner P, Billker O, Meyer TF et al.: "Nuclear factor-kappa B directs carcinoembryonic antigen-related cellular adhesion molecule 1 receptor expression in Neisseria gonorrhoeae-infected epithelial cells." in: J Biol Chem, Vol. 277, Issue 9, pp. 7438-46, 2002 (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid et al.: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, 2007 (PubMed).

Riesenberg, Weiler, Spring et al.: "Expression of indoleamine 2,3-dioxygenase in tumor endothelial cells correlates with long-term survival of patients with renal cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 23, pp. 6993-7002, 2007 (PubMed).

Kuespert, Roth, Hauck: "Neisseria meningitidis Has Two Independent Modes of Recognizing Its Human Receptor CEACAM1." in: PLoS ONE, Vol. 6, Issue 1, pp. e14609, 2011 (PubMed).