Stromal Cell Derived Factor 1 beta (SDF1b) antibody

Antigen: Stromal Cell Derived Factor 1 beta (SDF1b)

Synonyms: PBSF, SDF1, SDF1A, SDF1B, TPAR1, SCYB12, SDF-1a, SDF-1b, TLSF-a, TLSF-b, sdf1a, a-sdf1a

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN110541

Additional Information

Alternative name: SDF-1 beta (Stromal Cell-Derived Factor, PBSF)

Swiss-Prot: P48061

Immunogen: This polyclonal antibody, which reacts with both mouse and rat SDF-1b, was generated using E. coli-expressed mouse SDF-1b as an immunogen.

Format: Protein A purified, 0.1% sodium azide, Lyophilized

Isotype: IgG  (Matching secondary antibodies)

Description: SDF-1ß (stromal cell-derived factor-ß )1, also known as pre-B-cell growth-stimulating factor (PBSF)2, is a 74-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line1. Targeted deletion of SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis in mice3. SDF-1 has been shown to be chemotactic for lymphocytes4. In addition, SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells5,6., SDF-1 binding to CXCR4 inhibits HIV-1 entry5,6.

Specificity: Cross-reactivity to SDF-1b of other species has not been determined

Application Details

Application Notes: The tested titer for Western blot is 1:1,000, immunoprecipitation, 1:300-500; and immunohistochemistry, 1:100-500.

Concentration: 1mg/ml

Storage: 4 C for short term storage or -20C in small aliquots for long term storage. Avoid repeated freeze and thaw. Reconstitute to 1 mg/ml by adding 200 µ l PBS

Research Area: Immunology, Chemokines

Restrictions: For Research Use only

Images

Detection of rat recombinant SDF-1beta in transfected cells by Western Blot

Publications

Publications: Znalesniak, Duerer, Hoffmann: "Expression profiling of stationary and migratory intestinal epithelial cells after in vitro wounding: restitution is accompanied by cell differentiation." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 24, Issue 1-2, pp. 125-32, 2009 (PubMed).