YAF2 antibody (Middle Region)

Catalog No. ABIN1109525

The Rabbit Polyclonal anti-YAF2 antibody has been validated for WB and IHC (p). It is suitable to detect YAF2 in samples from Human, Mouse, Rat, Dog, Cow, Chicken and Xenopus laevis.

Quick Overview for YAF2 antibody (Middle Region) (ABIN1109525)

Target: YAF2 (YY1 Associated Factor 2 (YAF2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YAF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-YAF2 Antibody

Binding Specificity: Middle Region

Sequence: KGTSTRKPRP VSQLVAQQVT QQFVPPTQSK KEKKDKVEKE KSEKETTSKK

Cross-Reactivity (Details): Species reactivity (expected):Dog, Rat, Mouse, Bovine, Chicken, African clawed frogSpecies reactivity (tested):Human

Purification: Purified on Protein A affinity column

Immunogen: The immunogen for anti-YAF2 antibody: synthetic peptide directed towards the middle region of human YAF2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Add 100 μL of distilled water

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophised antibody at -20 °C for up to one year. Store reconstitued antibody undiluted for one month or in aliquots at -20 °C long term.

Expiry Date: 12 months

Target Details for YAF2

Target: YAF2 (YY1 Associated Factor 2 (YAF2))

Alternative Name: YAF2

Background: YAF2 interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. YAF2 contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1.The protein encoded by this gene interacts with YY1, a zinc finger protein involved in negative regulation of muscle-restricted genes. This gene product itself contains a single N-terminal C2-X10-C2 zinc finger, and in contrast to YY1, is up-regulated during myogenic differentiation. It also facilitates proteolytic cleavage of YY1 by the calcium- activated protease, m-calpain, suggesting a mechanism by which this protein antagonizes the negative effect of YY1. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two have been confirmed to date.Synonyms: YY1-associated factor 2

Gene ID: 10138

NCBI Accession: NP_005739