TUBB3 / TUBB4 (Loading Control) antibody (FITC)

Antigen: TUBB3 / TUBB4 (Loading Control)

Clonality: Monoclonal (TU-20)

Host: Mouse

Conjugate: FITC

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN110981

Additional Information

Characteristics: Synonyms: Tubulin beta-3 chain, Tubulin beta-III, Tubulin beta-4

Swiss-Prot: Q13509

Immunogen: Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin viacysteine added to the N-terminus of the neuron-specific peptideAA Sequence: ESESQGPK

Isotype: IgG1

Clone: TU-20

Description: The betaIII-tubulin isotype is present dominantly in cells of neuronal origin and it is one ofthe earliest marker of neuronal differentiation. Class III beta-tubulin, is regarded as aspecific probe for the cells of neuronal origin as well as for the tumours originating fromthese cells. The neuron-associated class III beta-tubulin isotype is most abundant in cellsof neuronal origin but was also detected in Sertoli cells of the testis and transiently innon-neuronal embryonic tissues.

Specificity: This antibody recognizes the C-terminal peptide sequence ESESQGPK (aa 441-448) ofhuman class III β-tubulin specific for neurones. MAb TU-20 is widely cross-reactive among species (recognized epitope conserved withinall species).

Application Details

Application Notes: Immunohistochemistry on Frozen and Paraffin Sections. Immunocytotochemistry on Fixed and Permeabilized cells. Suggested working dilution is 1/40. The conjugate was also successfully used on FFPE Sections using Confocal Microscopy. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS containing 15 mM Sodium Azide as preservative, approx. pH 7.4Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. Thereagent is free of unconjugated FITC.

Storage: Store the antibody in the dark at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Shelf life: one year from despatch.

Restrictions: For Research Use only