TUBB3 / TUBB4 (Loading Control) antibody

Antigen: TUBB3 / TUBB4 (Loading Control)

Clonality: Monoclonal (TU-20)

Host: Mouse

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN110982

Additional Information

Characteristics: Synonyms: Tubulin beta-3 chain, Tubulin beta-III, Tubulin beta-4

Swiss-Prot: Q13509

Immunogen: Peptide (C)441-448 coupled to maleimide-activated keyhole limpet hemocyanin viacysteine added to the N-terminus of the neuron-specific peptide.

Isotype: IgG1  (Matching secondary antibodies)

Clone: TU-20

Description: The beta-III Tubulin isotype is present dominantly in cells of neuronal origin and it is one ofthe earliest marker of neuronal differentiation. Class III beta-tubulin, is regarded as aspecific probe for the cells of neuronal origin as well as for the tumours originating fromthese cells. The neuron-associated class III beta-tubulin isotype is most abundant in cellsof neuronal origin but was also detected in Sertoli cells of the testis and transiently innon-neuronal embryonic tissues.

Specificity: The antibody recognizes the C-terminal peptide sequence ESESQGPK (aa 441-448) ofneuron-specific human betaIII-tubulin. Add. Information: The recognized epitope is conserved within all species.

Application Details

Application Notes: Flow cytometry. ELISA. Western Blotting: 1-2 µg/ml, 90 min. Positive Control: Porcine brain lysate. Negative Control: HPB-ALL peripheral blood leukemia cell line. Sample Preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 10 µg/mlStaining technique: Standard ABC technique (DAB+). Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl, incubation 30 min in RT or hightemperature citrate buffer antigen retrieval. Positive tissue: Neuronal tissue. Immunocytochemistry: FITC conjugate: 3 µg/ml. Positive Material: Neuro2a mouse neuroblastoma cell line. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS with 15 mM Sodium Azide, approx. pH 7.4

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only