Keratin 20 (KRT20) antibody

Antigen: Keratin 20 (KRT20)

Synonyms: K20, CD20, CK20, KRT21, MGC35423, 9030623C06Rik, Krt21, KRT20, MGC152269

Clonality: Monoclonal (KS20-3)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN111168

Additional Information

Alternative name: Cytokeratin 20

UniProt: P35900

Immunogen: Electrophoretically purified keratin K20 from humanintestinal mucosa.

Cross-Reactivity: Human, Pig (Porcine)

Isotype: IgG1

Clone: KS20-3

Description: Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinalepithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteinsresponsible for the structural integrity of epithelial cells and are subdivided intocytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which arearranged in pairs of heterotypic keratin chains. Their genes are clustered in a region ofchromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytesand goblet cells and is specifically expressed in the gastric and intestinal mucosa. It isalso expressed in adenocarcinomas of the colon, stomach, pancreas and the bile systemand is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, andendometrium, non-mucinous tumors of the ovary, and small cell carcinomas lackcytokeratin 20.

Characteristics: Synonyms: Cytokeratin 20, Keratin-20, KRT20, CK20, K20, Protein IT, Keratin type I cytoskeletal 20

Specificity: IT-Ks 20. 3 represents an excellent marker for certain types of carcinomas such asadenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel celltumors of the skin. Very sensitive detection of intestestinal and gastric foveolarepithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumorsoriginating therefrom (e. g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well asneuroendocrine tumors of the lung are essentially negative. Polypeptide recognized:

Application Details

Application Notes: Immunohistochemistry on frozen sections: 1: 10. Western Blot. Incubation Time: 1 h at RT or over night at 2-8C. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: Contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4Reconstitution: Restore in 1 ml dist. water

Storage: Stable for one year after reconstitution when stored at 2-8°C. For extended storage keep inaliquots at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix, Cancer

Restrictions: For Research Use only

Images

References

Publications

Moll, Loewe, Laufer et al.: "Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies." in: The American journal of pathology, Vol. 140, Issue 2, pp. 427-47, 1992 (PubMed).

Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).

Moll, Moll: "Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas." in: Archives of dermatological research, Vol. 283, Issue 5, pp. 300-9, 1991 (PubMed).

Moebus, Moll, Gerharz et al.: "Establishment of new ovarian and colon carcinoma cell lines: differentiation is only possible by cytokeratin analysis." in: British journal of cancer, Vol. 69, Issue 3, pp. 422-8, 1994 (PubMed).

Moll, Zimbelmann, Goldschmidt et al.: "The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas." in: Differentiation; research in biological diversity, Vol. 53, Issue 2, pp. 75-93, 1993 (PubMed).

Demirkesen, Hoede, Moll: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, 1996 (PubMed).

Harnden, Eardley, Joyce et al.: "Cytokeratin 20 as an objective marker of urothelial dysplasia." in: British journal of urology, Vol. 78, Issue 6, pp. 870-5, 1997 (PubMed).