clathrin, Heavy Chain (Hc) (CLTC) antibody

Antigen: clathrin, Heavy Chain (Hc) (CLTC)

Synonyms: Hc, CHC, CHC17, CLH-17, CLTCL2, KIAA0034, CLTC, Chc

Clonality: Monoclonal (CHC5-9)

Host: Mouse

Reactivity: Amphibian, Cow (Bovine), Human, Pig (Porcine), Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN111496

Additional Information

Characteristics: Synonyms: CLTC, CLH17, CLTCL2, KIAA0034, Membrane Vesicle Marker, Clathrin heavy chain onchromosome 17

Alternative name: Clathrin heavy chain

Gene ID: 281080

Swiss-Prot: P49951

Immunogen: Coated vesicles (Clathrin) of Bovine brain.

Isotype: IgM  (Matching secondary antibodies)

Clone: CHC5-9

Description: Clathrin is a protein which assembles into a polyhedral network on the cell membrane asthe membrane invaginates, forming a coated pit which is essential to endocytosis. Clathrinis composed of three polypeptides, a 180 kDa heavy chain and two 32-38 kDa light chainswhich combine to create a distinct three-legged triskelion. It is this morphology whichallows Clathrin to form its unique polyhedral network.

Specificity: Clone CHC5. 9 represents an excellent marker for detection of receptor mediatedendocytosis (for review cf. e. g. Refs. 2 and 3). Polypeptide Reacting: Mr 180 000 polypeptide (clathrin heavy chain) from coated vesiclesof various tissues. Structures and Tissues Specifically Detected: Coated vesicles of different organs and tissues (eg brain, mammary gland, ovaries). Reactivities on Cultured Cell Lines (tested so far): HeLa, SV-40, RVF-SMC.

Application Details

Application Notes: Immunohistochemistry on Frozen Tissue: 1/10. (When reconstituted, dilute further with PBS, pH 7.4). Incubation Time: 1 h at RT. Immunoblotting (Western): 1/100-1/500 (ECL). Successful use in Immunoprecipitation was reported by some researchers. As thisdetection method has not been verified by antibodies-online, the application was deletedfrom the database. This does not necessarily exclude the use in such procedure. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Storage: Store the antibody undiluted at 2-8°C. Shelf life: one year from despatch.

Research Area: Organelles, Transporters

Restrictions: For Research Use only

Publications

Publications: Bruder, Wiedenmann: "Identification of a distinct 9S form of soluble clathrin in cultured cells and tissues." in: Experimental cell research, Vol. 164, Issue 2, pp. 449-62, 1986 (PubMed).

Hirst, Robinson: "Clathrin and adaptors." in: Biochimica et biophysica acta, Vol. 1404, Issue 1-2, pp. 173-93, 1998 (PubMed).

Qualmann, Kessels, Kelly: "Molecular links between endocytosis and the actin cytoskeleton." in: The Journal of cell biology, Vol. 150, Issue 5, pp. F111-6, 2000 (PubMed).