Adenovirus (0) antibody

Antigen: Adenovirus (0)

Clonality: Monoclonal (143)

Host: Mouse

Application: Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Catalog no.: ABIN111660

Additional Information

Characteristics: Synonyms: Adenovirus antibody

Alternative name: Adenovirus

Immunogen: Infected cell extract with adenovirus type 6

Isotype: IgG  (Matching secondary antibodies)

Clone: 143

Description: Adenoviruses are DNA viruses generally widespread in nature that are frequently the causeof acute upper respiratory tract infections (i. e. common colds). Forty-seven knownserotypes have been isolated since they were first discovered in 1953 with 3 types knownto cause gastroenteritis. Several types have oncogenic potential though most causeself-limiting febrile illnesses characterised by inflammation of conjunctivae and therespiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgicallyremoved, indicating latent infections. It is not known how long the virus can persist in thebody, or whether it is capable of reactivation after long periods. In patients experiencingimmunosuppression (e. g. AIDS) it can be reactivated causing disease. The adenovirus early gene products E1A is a potent stimulator of cellular proliferation,which when overexpressed can overcome the growth inhibitory effects of TGF beta. The E1Aregion encodes a series of related proteins (35 - 46 kD) with multifunctional capabilitiesand forms a specific complex with the retinoblastoma tumor suppressor gene product. TheE1a and E1B regions together comprise the transforming region of adenovirus. Whileexpression of E1A alone is sufficient to immortalize primary cells, complete transformationrequires the additional expression of the E1B region.

Specificity: This Adenovirus antibody is specific for the penton or hexon group antigen of manyAdenovirus serotypes. Known reactivity with 34 serotypes of Adenovirus including types 40 and 41 (40, 41, 1, 2, 3,4, 5, 6, 7, 8, 9, 10, 11, 12, 14, 16, 18, 19, 20, 26, 31, 34, 35, 36 and 37

Application Details

Application Notes: Suitable for use in ELISA and IFA. Not suitable for use in Western blot. Recommended pairs for sandwich immunoassay: Capture DetectionBM3186 BM3301BM3186 ABIN126057Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml (OD 280 nm, E0.1% = 1.4)

Buffer: 0.01 M PBS, pH 7.2, containing 0.09% sodium azide as preservative and nostabilizers.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Virology

Restrictions: For Research Use only