Human Adenovirus type 6 (HAdV-6) antibody (FITC)

Catalog No. ABIN2859488

This Mouse Monoclonal antibody specifically detects Human Adenovirus type 6 in IF. It exhibits reactivity toward Human Adenovirus 6.

Quick Overview for Human Adenovirus type 6 (HAdV-6) antibody (FITC) (ABIN2859488)

Target: Human Adenovirus type 6 (HAdV-6)

Reactivity: Human Adenovirus 6

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Immunofluorescence (IF)

Clone: 143

Product Details

Immunogen: Infected cell extract with adenovirus type 6

Isotype: IgG

Application Details

Application Notes: Suitable for use in ELISA and IFA. Direct FA staining of target antigen in a permissive tissue culture system. Acetone fixation of the antigen source is recommended prior to staining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL (OD280 nm, E0.1% = 1.4)

Buffer: 0.01 M PBS, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.09 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions

Target Details

Target: Human Adenovirus type 6 (HAdV-6)

Alternative Name: Adenovirus Type 6

Target Type: Virus

Background: Adenoviruses are DNA viruses generally widespread in nature that are frequently the cause of acute upper respiratory tract infections (i.e. common colds). Forty-seven known serotypes have been isolated since they were first discovered in 1953 with 3 types known to cause gastroenteritis. Several types have oncogenic potential though most cause self-limiting febrile illnesses characterised by inflammation of conjunctivae and the respiratory tract. The virus can be isolated from the majority of tonsils/adenoids surgically removed, indicating latent infections. It is not known how long the virus can persist in the body, or whether it is capable of reactivation after long periods. In patients experiencing immunosuppression (e.g. AIDS) it can be reactivated causing disease. The adenovirus early gene products E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth inhibitory effects of TGF beta. The E1A region encodes a series of related proteins (35 - 46 kD) with multifunctional capabilities and forms a specific complex with the retinoblastoma tumor suppressor gene product. The E1a and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region.