Desmoglein 1/2 (DSG1/2) antibody

Details for Product No. ABIN111817
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Antigen
Synonyms DSG1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(3)
Host
Mouse
(3)
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(3), (3), (1), (1)
Pubmed 9 references available
Quantity 50 µg
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Catalog No. ABIN111817
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Immunogen "Band 3" polypeptide of isolated desmosomes from Bovine muzzle epidermis
Clone DG3-10
Isotype IgG1
Specificity This antibody recognizes Desmoglein 1+2 (desmosome-specific cadherins) in desmosome- bearing cells. It does not react with cells containing plaque-bearing junctions of non- desmosomal type. Tumors Specifically Detected: All epithelium-derived tumors, notably carcinomas, and
Purification Affinity Chromatography on Protein A
Alternative Name Desmoglein 1 + 2
Background Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 and 2 are calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18.
Alternate names: CDHF4, CDHF5, DG1, DGI, DSG-1, DSG-2, DSG1, DSG2, Desmoglein1, Desmoglein2, Desmosomal glycoprotein 1, HDGC, Pemphigus foliaceus antigen
Gene ID 1829
NCBI Accession NP_001934
UniProt Q14126
Research Area Cytoskeleton
Application Notes Western blotting. Immunofluorescence Microscopy. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Tissue (after microwave treatment). Working Dilution: Dilute at least 1/10 with PBS, pH 7.4, for Immunohistochemicalapplication. Incubation time: 1 h at RT, extended with paraffin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1.0 mL distilled water.
Buffer Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Koch, Walsh, Schmelz et al.: "Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules." in: European journal of cell biology, Vol. 53, Issue 1, pp. 1-12, 1991 (PubMed).

Yang, Shaio, Wang et al.: "Neuroblastoma cell-mediated leukocyte chemotaxis: lineage-specific differentiation of interleukin-8 expression." in: Experimental cell research, Vol. 211, Issue 1, pp. 1-5, 1994 (PubMed).

Schmelz, Franke: "Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes." in: European journal of cell biology, Vol. 61, Issue 2, pp. 274-89, 1993 (PubMed).

Schäfer, Stumpp, Franke: "Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues." in: Differentiation; research in biological diversity, Vol. 60, Issue 2, pp. 99-108, 1996 (PubMed).

Hatzfeld, Haffner, Schulze et al.: "The function of plakophilin 1 in desmosome assembly and actin filament organization." in: The Journal of cell biology, Vol. 149, Issue 1, pp. 209-22, 2000 (PubMed).

Caldelari, de Bruin, Baumann et al.: "A central role for the armadillo protein plakoglobin in the autoimmune disease pemphigus vulgaris." in: The Journal of cell biology, Vol. 153, Issue 4, pp. 823-34, 2001 (PubMed).

Akat, Mennel, Kremer et al.: "Molecular characterization of desmosomes in meningiomas and arachnoidal tissue." in: Acta neuropathologica, Vol. 106, Issue 4, pp. 337-47, 2003 (PubMed).

Bosch, Andl, Abel et al.: "E-cadherin is a selective and strongly dominant prognostic factor in squamous cell carcinoma: a comparison of E-cadherin with desmosomal components." in: International journal of cancer. Journal international du cancer, Vol. 114, Issue 5, pp. 779-90, 2005 (PubMed).

Ponte-Sucre, Moll: "Parasites, flies and men--21st Meeting of the German Society of Parasitology in Würzburg." in: European journal of cell biology, Vol. 84, Issue 1, pp. 7-13, 2005 (PubMed).

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