Desmoplakin (DSP) antibody

Antigen: Desmoplakin (DSP)

Synonyms: DPI, DPII, DSP, dpi, dpii, DP, AA407887, AA407888, AW109828, 2300002E22Rik, 5730453H04Rik, wu:fc17a08, wu:fk73d01

Clonality: Monoclonal (DP2-15)

Host: Mouse

Reactivity: Amphibian, Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN111818

Additional Information

Characteristics: Synonyms: DP, DSP, 250/210 kDa paraneoplastic pemphigus antigen

Alternative name: Desmoplakin

Gene ID: 1832

Swiss-Prot: P15924

Immunogen: Bovine desmoplakin 1&2

Isotype: IgG1  (Matching secondary antibodies)

Clone: DP2-15

Description: Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attachesintermediate filaments to desmosomal plaques. It is involved in the organization ofdesmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome andinteraction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds tointermediate filaments. The central region of desmoplakin comprises a coiled-coil roddomain that mediates homodimerisation. There are two isoforms of desmoplakin -desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II,which is predominantly expressed in tissues and cells of stratified origin. Mutations in thegene encoding desmoplakin result in a number of cardiomyopathies and keratodermas aswell as the autoimmune disease paraneoplastic pemphigus.

Specificity: This antibody reacts to Desmoplakin 1&2. It shows distinct punctate membrane staining of different epithelia. Tumors specifically detected: Primary and metastatic carcinoma and meningioma.

Application Details

Application Notes: Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections: Dilute 1/10 with PBS, pH 7.4 (Incubation Time: 1 h at RT). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: Final solution contains: PBS, pH 7.4 with 0.09% Sodium Azide aspreservative and 0.5% BSA as stabilizer.Reconstitution: Restore in 1 ml dist. water.

Storage: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cytoskeleton

Restrictions: For Research Use only