Desmoplakin (DSP) antibody

Details for Product No. ABIN111818
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Antigen
Synonyms DSP, dpi, dpii, im:6911953, sb:cb570, wu:fc17a08, wu:fk73d01, DP, DPI, DPII, 2300002E22Rik, 5730453H04Rik, AA407887, AA407888, AW109828, fi04e12
Reactivity
Amphibian, Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)
(33), (17), (15), (14), (12), (8), (2), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(24), (6), (6), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(20), (14), (14), (10), (9), (8), (3), (2), (2)
Pubmed 10 references available
Quantity 50 µg
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Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN111818
412.50 $
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Immunogen Bovine Desmoplakin 1 and 2
Clone DP2-15
Isotype IgG1
Specificity This antibody reacts to Desmoplakin 1&2. It shows distinct punctate membrane staining of different epithelia. Tumors Specifically Detected: Primary and metastatic carcinoma and meningioma. Polypeptides Reacting: Desmoplakin 1 and 2 (Mr 250 000 and 215 000). Reactivity on Tested Cell Lines: Several Human carcinoma cell lines: MCF-7, A-431, Rat MHC1C1 cell line, Bovine cells: MDBK, BMGE. Species Reactivity: Tested: Human, Bovine, Rat, Mouse and Chicken.
Purification Affinity Chromatography on Protein A
Alternative Name Desmoplakin
Background Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.
Alternate names: 250/210 kDa paraneoplastic pemphigus antigen, DP, DSP
Gene ID 1832
NCBI Accession NP_001008844
UniProt P15924
Research Area Cytoskeleton
Application Notes Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections: Dilute 1/10 with PBS, pH 7.4 (Incubation Time: 1 h at RT). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore in 1 mL dist. water.
Buffer Final solution contains PBS, pH 7.4 0.09% Sodium Azide 0.5% BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody (in aliquots) at -20°C.
Expiry Date 12 months
Background publications Koch, Walsh, Schmelz et al.: "Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules." in: European journal of cell biology, Vol. 53, Issue 1, pp. 1-12, 1991 (PubMed).

Moll, Moll: "Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas." in: Archives of dermatological research, Vol. 283, Issue 5, pp. 300-9, 1991 (PubMed).

Owaribe, Nishizawa, Franke: "Isolation and characterization of hemidesmosomes from bovine corneal epithelial cells." in: Experimental cell research, Vol. 192, Issue 2, pp. 622-30, 1991 (PubMed).

Cowin, Kapprell, Franke: "The complement of desmosomal plaque proteins in different cell types." in: The Journal of cell biology, Vol. 101, Issue 4, pp. 1442-54, 1985 (PubMed).

Moll, Cowin, Kapprell et al.: "Desmosomal proteins: new markers for identification and classification of tumors." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 54, Issue 1, pp. 4-25, 1986 (PubMed).

Dockhorn-Dworniczak, Franke, Schröder et al.: "Patterns of expression of cytoskeletal proteins in human thyroid gland and thyroid carcinomas." in: Differentiation; research in biological diversity, Vol. 35, Issue 1, pp. 53-71, 1988 (PubMed).

Franke, Moll, Mueller et al.: "Immunocytochemical identification of epithelium-derived human tumors with antibodies to desmosomal plaque proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 2, pp. 543-7, 1983 (PubMed).

Schmelz, Franke: "Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes." in: European journal of cell biology, Vol. 61, Issue 2, pp. 274-89, 1993 (PubMed).

Hatzfeld, Haffner, Schulze et al.: "The function of plakophilin 1 in desmosome assembly and actin filament organization." in: The Journal of cell biology, Vol. 149, Issue 1, pp. 209-22, 2000 (PubMed).

Bosch, Andl, Abel et al.: "E-cadherin is a selective and strongly dominant prognostic factor in squamous cell carcinoma: a comparison of E-cadherin with desmosomal components." in: International journal of cancer. Journal international du cancer, Vol. 114, Issue 5, pp. 779-90, 2005 (PubMed).

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