F4/80 antibody (APC)

Catalog No. ABIN111848

The Rat Monoclonal anti-F4/80 antibody is suitable to detect F4/80 in samples from Mouse. It has been validated for FACS.

Quick Overview for F4/80 antibody (APC) (ABIN111848)

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This F4/80 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: CI:A3-1

Product Details anti-F4/80 Antibody (APC)

Specificity: This antibody recognises the Mouse F4/80 antigen, a 160 kDa glycoprotein expressed by murine macrophages. The antibody binds to Macrophages from different sites including the peritoneal cavity, lung, spleen and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. The antigen is not expressed by lymphocytes or polymorphonuclear cells.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein A .

Immunogen: Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS-1 mouse myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water.

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA 5 % sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!

Target Details for F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Alternative Name: Macrophage F4/80 Antigen

Background: F4/80 antigen is a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with Human EGF module-containing Mucin-like Hormone Receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480

Gene ID: 13733

NCBI Accession: NP_034260

UniProt: Q61549