F4/80 antibody (Biotin)

Catalog No. ABIN111849

The Biotin-conjugated Rat Monoclonal anti-F4/80 antibody (Clone Cl:A3-1) (ABIN111849) specifically detects F4/80 in FACS, WB, IHC (p) and IHC (fro). The antibody is reactive with Mouse samples.

Quick Overview for F4/80 antibody (Biotin) (ABIN111849)

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This F4/80 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Cl:A3-1

Product Details anti-F4/80 Antibody (Biotin)

Specificity: This antibody recognizes the Murine F4/80 antigen, a 160 kDa cell surface glycoprotein. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (Ref.5).

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein G.

Immunogen: Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat to 1/10 diluted antibody to stain 10^6 cells in 100 μL. Immunohistology on Frozen and Paraffin Embedded Sections: For paraformaldehydeparaffin tissue, pretreatment by trypsinisation for 20-30 minutes is required. Use 1/50 oncryosections, 1/20 on paraffin sections. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Alternative Name: Macrophage F4/80 Antigen

Background: F4/80 antigen is a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with human EGF module-containing mucinlike hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480

Gene ID: 13733

NCBI Accession: NP_034260

UniProt: Q61549